ORDERINGINFORMATION
Catalognos.:56336-50(50ug)56336-200(200ug)
Format:ProteinA-purifiedantibodyinPBS,50%glycerol,0.09%sodiumazide,.
BACKGROUND
CalcineurinisaCa+2/calmodulin-dependentserine/threonineproteinphosphatase.TheactiveenzymeisaheterodimerconsistingofacatalyticAsubunitandamyristylatedregulatoryBsubunitthatbindscalcium.ItcontrolsthetranslocationofnuclearfactorsfromthecytosolintothenucleusofactivatedTcells.CalcineurinisatargetofimmunosuppressivedrugssuchascyclosporinAandFK506.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingtoaa264-283ofhumanCalcineurinAconjugatedtoKLH.ThissequenceisidenticalinratandmousecalcineurinA.
Accessionno.:P16298
GeneID:5532
HostSpecies:Rabbit
AntibodyClass:Polyclonal
Specificity:Thisantibodyrecognizeshuman,mouse,rat,rabbit,canine,bovine,porcine,chicken,monkey,hamster,andDrosophilaCalcineurinA.
APPLICATIONS
Immunoblotting:Useat1-5ug/ml.Abandof~61kDaisdetected.
DetectionofCalcineurinAinratbrainlysate(Lane1)andmousebrainlysate(Lane2).
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze/thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
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细胞质中有各种细胞器,细胞内的化学反应大多都是在细胞质中进行,因此是代谢中心.
细胞核中有DNA,是遗传信息库,是细胞代谢和遗传的控制中心.
解析：1.细胞质基质（也叫胞质溶胶）是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是：细胞核、线粒体基质、叶绿体基质等基质。
不破坏细胞结构，同步动态侦测细胞有氧呼吸，糖酵解OCR/ECA(总或乳酸ECA)；
i.过氧敏感荧光素（或pH敏感荧光素）,Ex340-380/535/Em630-680nm，实时测量线粒体/胞内/胞外重要代谢指标，荧光素为非结构性结合可逆转改变；
ii.可同时或单个测量OCR/ECA或其他参数，不会做成浪费，可采用时间分辨荧光技术可加强系统信噪比
iii.通过两个加药口，可对检测细胞加入适当抑制剂/刺激实时监测细胞对不同毒素作用
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