Graphene oxide is one of the most popular 2D materials available. This is due to the wide range of fields that it can be applied to. It has a distinct advantage over other 2d materials (such as graphene), as it is easily dispersed within solution; allowing for processing at high concentrations. This has opened it up for use in applications such as optical coatings, transparent conductors, thin-film batteries, chemical resistant coatings, water purification, and many more. Ossila have two types of graphene oxide powders available, with flake sizes between 1-5um and 1-50um. In addition, we also offer pre-dispersed graphene oxide solutions for simple instant use. |
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Product List
MSDS 
Graphene Oxide Powders
| Product code | M880 | M881 | M882 |
| Flake Size | 100 - 200nm | 1-5 μm | 1-50 μm |
| Flake Thickness | 0.8 - 1.2 nm | 0.8-1.2 nm | 0.8-1.2 nm |
| Single layer ratio | >99% | >99% | >99% |
| Purity | >99% | >99% | >99% |
| Amount | 100mg, 500mg | 500mg, 1g | 500mg, 1g |
| Packaging Information | Light resistant bottle | Light resistant bottle | Light resistant bottle |
MSDS 
Graphene Oxide Solutions
| Product code | M883 | M884 | M885 | M886 |
| Flake Sizes | 1-5 μm | 1-5 μm | 1-50 μm | 1-50 μm |
| Concentration | 5 mg.ml-1 | 0.5 mg.ml-1 | 5 mg.ml-1 | 0.5 mg.ml-1 |
| Solvents | Water:IPA | Water:IPA | Water:IPA | Water:IPA |
| Solution Volume | 100 ml | 100 ml | 100 ml | 100 ml |
| Packaging Information | 4 x 25 ml bottles | 4 x 25 ml bottles | 4 x 25 ml bottles | 4 x 25 ml bottles |
What Graphene Oxide is
Graphene oxide (GO), also referred to as graphite/graphitic oxide, is obtained by treating graphite with oxidisers, and results in a compound of carbon, oxygen, and hydrogen in variable ratios.
The structure and properties of GO are much dependent on the particular synthesis method and degree of oxidation. With buckled layers and an interlayer spacing almost two times larger (~0.7 nm) than that of graphite,it typically still preserves the layer structure of the parent graphite.
GO absorbs moisture proportionally to humidity and swells in liquid water. GO membranes are vacuum-tight and impermeable to nitrogen and oxygen, but permeable to water vapours. The ability to absorb water by GO depends on the particular synthesis method and also shows a strong temperature dependence.
GO is considered as an electrical insulator for the disruption of its sp2 bonding networks. However, by manipulating the content of oxygen-containing groups through either chemical or physical reduction methods, the electrical and optical properties of GO can be dynamically tuned. To increase the conductivity, oxygen groups are removed by reduction reactions to reinstall the delocalised hexagonal lattice structure. One of the advantages GO has over graphene is that it can be easily dispersed in water and other polar organic solvents. In this way, GO can be dispersed in a solvent and reduced in situ, resulting in potentially monodispersed graphene particles.
Due to its unique structure, GO can be functionalised in many ways for desired applications, such as optoelectronics, drug delivery, chemical sensors, membrane filtration, flexible electronics, solar cells and more.
GO was first synthesised by Brodie (1859), followed by Hummers" Method (1957), and later on by Staudenmaier and Hofmann methods. Graphite (graphene) oxide has also been prepared by using a "bottom-up" synthesis method (Tang-Lau method) where glucose is the sole starting material. The Tang-Lau method is considered to be easier, cheaper, safer and more environmentally-friendly. The thickness, ranging from monolayer to multilayers, can by adjusted using the Tang-Lau process. The effectiveness of an oxidation process is often evaluated by the carbon/oxygen ratios of the GO.
Dispersion Guides
Due to the presence of oxygen and hydroxide groups, the dispersibility of this material is significantly better than other 2d materials (such as graphene). High concentrations of GO can be dispersed in polar solvents, such as water. At Ossila, we have found that the most stable solutions can be produced using the following recipe:
- Weigh out desired amount of material, this can go up to at least 5 mg.ml-1.
- Add 1:1 ratio of deionized water to isopropyl alcohol.
- Shake vigorously to break up material.
- A short treatment in an ultrasonic bath will rapidly disperse the material.
- For larger flakes, use a mechanical agitator instead (as sonication may damage the flakes).
Technical Data
General Information
| CAS number | 7782-42-5 (graphite) |
| Chemical formula | CxHyOz |
| Recommended Solvents | H2O, DMF, IPA |
| Synonyms |
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| Classification / Family | 2D semiconducting materials, Carbon nanomaterials, Graphene, Organic electronics |
| Colour | Black/Brown Sheets/Powder |
Product Images
Publications
- An improved Hummers method for eco-friendly synthesis of graphene oxide, J. Chen et al., Carbon 64, 225-229 (2013); http://dx.doi.org/10.1016/j.carbon.2013.07.055.
- Synthesis of few-layered, high-purity graphene oxide sheets from different graphite sources for biology, D. A. Jasim et al., 2D Mater. 3, 014006 (2016); doi:10.1088/2053-1583/3/1/014006.
- Preparation and Characterization of Graphene Oxide, J. Song et al., J. Nanomater., 276143 (2014); http://dx.doi.org/10.1155/2014/276143.
- The chemistry of graphene oxide, D. R. Dreyer et al., Chem. Soc. Rev., 39, 228–240 (2010); DOI: 10.1039/b917103g.
- Preparation of small-sized graphene oxide sheets and their biological applications, M. Zhang et al., J. Mater. Chem. B, 4, 121 (2016); DOI: 10.1039/c5tb01800e.
- Graphene Oxide: Preparation, Functionalization, and Electrochemical Applications, D. Chen et al., Chem. Rev., 112, 6027−6053 (2012); dx.doi.org/10.1021/cr300115g.
- Preparation of Graphitic Oxide, W. Hummer et al., J. Am. Chem. Soc., 80 (6), 1339–1339 (1958); DOI: 10.1021/ja01539a017.
- Improved Synthesis of Graphene Oxide, D. C. Marcano et al., ACS Nano, 4 (8), 4806–4814 (2010); DOI: 10.1021/nn1006368.
- Fast and fully-scalable synthesis of reduced graphene oxide, S. Abdolhosseinzadeh et al., Sci. Rep., 5:10160 (2015); DOI: 10.1038/srep10160.
To the best of our knowledge, the technical information provided here is accurate. However, Ossila assume no liability for the accuracy of this information. The values provided here are typical at the time of manufacture and may vary over time and from batch to batch.
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TBST中含有Tris-Hcl，NaCl，Tween20这三种物质，是做WESTERNBLOT中常用的一种缓冲液。
TBST缓冲液的配制
1000ml×TBST的配置
先称量NaCl40g，倒入烧杯中，加DDW蒸馏水400ml,再称量NaCl47.6g，倒入刚才的那个烧杯中（PS：由于NaCl的量太多，一次称量不方便，所以分两次称量，且易于溶解）。往烧杯中加入Tris—HCl缓冲液100ml，最后加（吐温20）5ml,转入1000ml容量瓶中，在定容，转移即可。
TBST缓冲液的应用：
1.主要用于免疫组化和原位杂交，酶联免疫等实验中，清洗免疫印。
2.迹膜；
注意事项：
1.TBST缓冲液，PH7.2-7.5；
2.颜色为无色透明液体；
3.为了您的安全和健康，请穿实验服并戴防护手套操作；
碳酸氢钠溶液是一种co2缓冲液,当瓶内二氧化碳量减少时,碳酸氢钠溶液释放co2,反之吸收co2.因此它能保持瓶内二氧化碳量大致不变.
1、组成成分：
A、1×TE缓冲液：10mmol/LTris.Cl；1mmol/LEDTA，pH8.0。
B、1×TAE缓冲液：40mmol/LTris-乙酸；2mmol/LEDTA，pH8.0。
C、1×TBE缓冲液：45mmol/LTris-硼酸；1mmol/LEDTA，pH8.0。
2、用途：
A、TE缓冲液：一般用作溶解剂或保持剂，常用于溶解DNA，能稳定储存DNA。
B、TAE缓冲液:生物学中使用最广泛的核酸电泳缓冲液，主要用于DNA的琼脂糖凝胶电泳。
C、TBE缓冲液：生物学中常用的核酸电泳缓冲液，主要用于DNA的琼脂糖凝胶电泳。
3、TAE和TBE缓冲液的选择：
TAE和TBE缓冲液都可以用于DNA的琼脂糖凝胶电泳，两者各有利弊，应根据实际情况选择不同的缓冲液。
4、注意：
TBE浓溶液长时间存放后会形成沉淀物，出现沉淀后应予以废弃。
求采纳为满意回答。
1 mmol/LEDTA(pH 8.0)
因为含有以上两种物质，所以称为TE。
配制分三步：
1)1 M Tris-HCl (pH 8.0) 50 ml的配制：称取Tris碱6.06 g，加超纯水40 ml溶解，滴加浓HCl约2.1 ml调pH至8.0，定容至50 ml。
2)0.5 M EDTA（pH 8.0）50 ml的配制：称取EDTA-Na2·2H2O 9.306 g，加超纯水35 ml，剧烈搅拌，用约1 g NaOH颗粒调pH至8.0，定容至50 ml。（EDTA二钠盐需加入NaOH将pH调至接近8.0时，才会溶解。）
3)1×TE（10 mM Tris-HCl，pH 8.0；1 mM EDTA，pH 8.0）的配制：
作用：
TE缓冲液是弱碱性,对DNA的碱基有保护性,(DNA在它是的稳定性较好,不易破坏其完整性或产生开环及断裂),包括提取好的DNA也要放在TE缓冲液是保存. 10mMTris-Hcl，pH有7.47.68.0三种。
EDTA调到8.0是为了更好溶解，其他只要调到相应pH就可以。Tris在7－8附近缓冲能力很强，所以加8.0的EDTA下去后，不会改变pH。
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