DesaltingandBufferExchangeProcedure
1.SelectthemostappropriateMWCOforyoursample.Formaximumrecovery,selectaMWCO1to2themolecularsizeofthespeciesofinterest.
2.Fillconcentratorwithuptothemaximumvolumestatedinthedeviceoperatinginstructions*,(e.g.20mlifVivaspin?20isused).
3.Ifthesampleissmallerthanthemaximumdevicevolume*,itcanbediluteduptothe
maximumvolumebeforethefirstcentrifugationstep.Thiswillhelpincreasethesaltremovalrate.
4.CentrifugefortherecommendedamountoftimeatanappropriatespinspeedforyourVivaspin?model*.
5.Emptyfiltratecontainer?.
6.Refillconcentratorwithanappropriatesolvent.
7.Centrifugeagainasbefore.
8.Emptyfiltratecontainer?.
9.Recovertheconcentrated,de-saltedsamplefromthebottomoftheconcentratepocketwithaPipette.