TakaraPODconjugatesaredesignedtoreplacetrADItionalsecondaryantibodiesinimmunohistochemicalstainingofparaffin-embeddedtissuesections.Usetheseproductsforasimplerandeasiermethodthanthosebasedonstreptavidin-biotin,andobtainclear,highlysensitiveresultswithminimalbackground.EachTakaraPODconjugateconsistsofanaminoacidpolymercombinedwithperoxidaseandtheFab"fragmentsofasecondaryantibody.Thelabeledpolymersbindtotheprimaryantibody-antigentoformanHRP-labeledcomplex.Thecomplexisvisualizedbytheadditionofacolorimetricsubstrate,suchasDABSubstrate(seebelow).
TakaraPODconjugatesaredesignedtoreplacetraditionalsecondaryantibodiesinimmunohistochemicalstainingofparaffin-embeddedtissuesections.Usetheseproductsforasimplerandeasiermethodthanthosebasedonstreptavidin-biotin,andobtainclear,highlysensitiveresultswithminimalbackground.
EachTakaraPODconjugateconsistsofanaminoacidpolymercombinedwithperoxidaseandtheFab"fragmentsofasecondaryantibody.Thelabeledpolymersbindtotheprimaryantibody-antigentoformanHRP-labeledcomplex.Thecomplexisvisualizedbytheadditionofacolorimetricsubstrate.
Thisproductisavailableintwodifferentformats,formousetissuesections("ForMouseTissue")andfortissuesectionsfromothermammaliansources("ForTissue").Specificversionsareavailablebasedonthesourcespeciesoftheprimaryantibody;"AntiMouse","AntiRat",and"AntiRabbit"versionsareavailableformousetissuesections,and"AntiMouse","AntiRabbit",and"AntiGoat"versionsareavailableformammaliantissue.
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这样子好吗?我个人认为理论上问题不大,不知道是不是
我现在做药物动力学中的组织分布部分试验,现在遇到了难题,就是肝脏组织太脏,不知道该如何处理才能上样呢?谢谢!!
还有就是想请教各位老师,小鼠尾静脉注射有什么技巧呢?我有时候能打进去,有时候打到组织中啦,谢谢各位大师指点迷津。
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