Thiskitprovidesareagentsettoallowsimple,rapiddetectionofenterohemorrhagicE.coli(EHEC)byamplificationoftheverocytotoxingeneusingPCR.IthasbeendesignedforscreeningofsevenmajorSEROtypeswithintheO-antigentypes(O26,O103,O111,O121,O145,O157,andO165).AmultiplexPCRenzymemix,2XPCRbuffer(withMg2+anddNTP),primermix,andcontrolsaresuppliedwiththekit.
Thiskitprovidesareagentsettoallowsimple,rapiddetectionofenterohemorrhagicE.coli(EHEC)byamplificationoftheverocytotoxingeneusingPCR.IthasbeendesignedforscreeningofsevenmajorserotypeswithintheO-antigentypes(O26,O103,O111,O121,O145,O157,andO165).AmultiplexPCRenzymemix,2XPCRbuffer(withMg2+anddNTP),primermix,andcontrolsaresuppliedwiththekit.Additionally,thiskitallowsforsimultaneousdetectionofverocytotoxintype1,verocytotoxintype2,andtheeaegene.ThisproductisdesignedforanalysisofDNAextractedfromfoodsamplesusingthehotalkalineextractionmethod.AfterrunningmultiplexPCR,samplesaresubjectedtogelelectrophoresis,allowingvisualizationofOantigens,verocytotoxin,eaegene,andinternalcontrolbands.Note:AssociateProfessorAtsushiIguchioftheDepartmentofAnimalandGrasslandSciences,FacultyofAgriculture,UniversityofMiyazakicontributedtothedevelopmentofthiskit.
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抗原是指一种能刺激人或动物机体产生抗体或致敏淋巴细胞,并能与这些产物在体内或体外发生特异性反应的物质。抗原的基本能力是免疫原性和反应原性。免疫原性又称为抗原性,是指能够刺激机体形成特异抗体或致敏淋巴细胞的能力。反应原性是指能与由它刺激所产生的抗体或致敏淋巴细胞发生特异性反应。具备免疫原性和反应原性两种能力的物质称为完全抗原,如病原体、异种动物血清等。只具有反应原性而没有免疫原性的物质,称为半抗原,如青霉素、磺胺等。半抗原没有免疫原性,不会引起免疫反应。但在某些特殊情况下,如果半抗原和大分子蛋白质结合以后,就获得了免疫原性而变成完全抗原。
一个制造一种专一抗体的浆细胞进行培养,就可得到由单细胞经分裂增殖而形成细胞群,即单克隆。单克隆细胞将合成针对一种抗原决定簇的抗体,称为单克隆抗体。
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