Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.
Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6 kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.
Advantage 2 Polymerase Mix is perfect for PCR applications requiring accuracy, such as RACE PCR, cDNA amplification, library construction, and PCR subtraction. Advantage 2 Polymerase Mix is highly recommended for applications downstream of our SMART(er) method for cDNA synthesis.
Advantage 2 Polymerase Mix is also available in a complete PCR kit that includes dNTPs and PCR controls, or in a ready-to-use, lyophilized format (High Fidelity PCR EcoDry Premix).
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请赐教!
荚膜(capsule)是某些细菌在生长繁殖过程中分泌的一层黏液性物质,包围在细胞壁外,通常这种黏液层厚度小于0.2μm,成分是多糖或多肽,只有在营养丰富时或在动物体内,细菌才产生这种半抗原性质的黏液性物质。它具有保护菌体免受巨噬细胞等的捕捉和吞噬,因而具有抗吞噬抗消化、侵袭力强、与致病性关系密切等特点。像肺炎球菌、炭疽杆菌等都有这类荚膜。有些细菌的荚膜层较薄,小于0.2μm,称为微荚。
像链球菌的M蛋白、伤寒杆菌的Vi抗原、大肠杆菌的K抗原等都属于这类微荚膜。
荚膜是某些细菌在细胞壁外包围的一层粘液性物质,一般由糖和多肽组成,是细菌的一种特殊结构。
作用:
①抗吞噬作用:荚膜因其亲水性及其空间占位、屏障作用,可有效抵抗寄主吞噬细胞的吞噬作用。
②黏附作用:荚膜多糖可使细菌彼此间粘连,也可黏附于组织细胞或无生命物体表面,是引起感染的重要因素,具有荚膜的S-型肺炎链球菌毒力强,有助于肺炎链球菌侵染人体;废水生物处理中的细菌荚膜有生物吸附作用,将废水中的有机物、无机物及胶体吸附在细菌体表面上。
③抗有害物质的损伤作用:处于细菌细胞最外层,荚膜犹如盔甲可有效保护菌体免受或少受多种杀菌、抑菌物质的损伤,如溶菌酶、补体等。
④抗干燥作用:荚膜多糖为高度水合分子,含水量在95%以上,可帮助细菌抵抗干燥对生存的威胁。
⑤当缺乏营养时,荚膜可被利用作碳源和能源,有的荚膜还可作氮源。
所以一个细菌或病毒可以有很多的抗原。
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