pRS316载体
| 货号： | ZY77145 |
| 供应商： | 泽叶生物 |
| 数量： | 20 |
| 英文名： | pRS316 |
| 保质期： | 三年 |
| 保存条件： | -20℃低温保存 |
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRS316 4887 bp ds-DNA circular SYN 09-九月-2012DEFINITION Yeast centromere vector with URA3 Marker.ACCESSIONU03442VERSION.KEYWORDSpRS316SOURCEsyntheticDNAconstructORGANISMsyntheticDNAconstructREFERENCE1(bases1to4887)AUTHORSSikorskiRS,HieterP.TITLEAsystemofshuttlevectorsandyeasthoststrainsdesignedforefficientmanipulationofDNAinSaccharomycescerevisiae.JOURNALGenetics1989;122:19-27.PUBMED2659436REFERENCE2(bases1to4887)AUTHORS.TITLEDirectSubmissionJOURNALExported2013年8月13日fromSnapGeneViewer1.5.3http://www.snapgene.comCOMMENTFEATURESLocation/Qualifierssource1..4887/organism="syntheticDNAconstruct"/lab_host="Saccharomycescerevisiae"/mol_type="otherDNA"promoter190..410/gene="S.cerevisiaeURA3"/note="URA3promoter"/note="color:#ffffff;direction:RIGHT"CDS411..1214/codon_start=1/gene="S.cerevisiaeURA3"/product="orotidine-5-phosphatedecarboxylase,requiredforuracilbiosynthesis"/note="URA3"/note="yeastauxotrophicmarker,counterselectablewith5-fluorooroticacid(5-FOA)"/note="color:#ff7f50"/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"rep_origincomplement(1343..1798)/direction=LEFT/note="f1ori"/note="f1bacteriophageoriginofreplication;arrowindicatesdirectionof(+)strandsynthesis"/note="color:#ffff00"CDScomplement(1581..2156)/codon_start=1/gene="lacZ?fragment"/product="fragmentof?beta-galactosidase"/note="lacZ"/note="color:#993366"/translation="MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLVLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"primer_bind1943..1959/note="M13fwd"/note="commonsequencingprimer,oneofmultiplesimilarvariants"/note="color:#a020f0;direction:RIGHT"promoter1966..1984/note="T7promoter"/note="promoterforbacteriophageT7RNApolymerase"/note="color:#ffffff;direction:RIGHT"misc_feature1993..2100/note="MCS"/note="pBluescriptmultiplecloningsite"/note="color:#99ccff"promotercomplement(2113..2131)/note="T3promoter"/note="promoterforbacteriophageT3RNApolymerase"/note="color:#ffffff;direction:LEFT"primer_bindcomplement(2152..2168)/note="M13rev"/note="commonsequencingprimer,oneofmultiplesimilarvariants"/note="color:#a020f0;direction:LEFT"protein_bind2176..2192/bound_moiety="lacrepressorencodedbylacI"/note="lacoperator"/note="ThelacrepressorbindstothelacoperatortoinhibittranscriptioninE.coli.Thisinhibitioncanberelievedbyaddinglactoseorisopropyl-beta-D-thiogalactopyranoside(IPTG)."/note="color:#31849b"promotercomplement(2200..2230)/note="lacpromoter"/note="promoterfortheE.colilacoperon"/note="Thisreversedirectionalfeaturehas3segments:???1:?2200?..?2206?/?#ffffff?/?-10???2:?2207?..?2224?/?#ffffff???3:?2225?..?2230?/?#ffffff?/?-35"rep_origincomplement(2554..3142)/direction=LEFT/note="ori"/note="high-copy-numbercolE1/pMB1/pBR322/pUCoriginofreplication"/note="color:#ffff00"CDScomplement(3313..4173)/codon_start=1/gene="bla"/product="beta-lactamase"/note="AmpR"/note="confersresistancetoampicillin,carbenicillin,andrelatedantibiotics"/note="Thisfeaturehas2segments:???1:?3313?..?4104?/?#ccffcc???2:?4105?..?4173?/?#ccffcc?/?signal?sequenceCleavagesiteafterbase4104"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promotercomplement(4174..4278)/gene="bla"/note="AmpRpromoter"/note="color:#ffffff;direction:LEFT"misc_feature4315..4818/note="CEN/ARS"/note="S.cerevisiaeCEN6centromerefusedtoanautonomouslyreplicatingsequence"/note="color:#454f59"ORIGIN1tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtca61cagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtg121ttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgc181accacgcttttcaattcaattcatcattttttttttattcttttttttgatttcggtttc241tttgaaatttttttgattcggtaatctccgaacagaaggaagaacgaaggaaggagcaca301gacttagattggtatatatacgcatatgtagtgttgaagaaacatgaaattgcccagtat361tcttaacccaactgcacagaacaaaaacctgcaggaaacgaagataaatcatgtcgaaag421ctacatataaggaacgtgctgctactcatcctagtcctgttgctgccaagctatttaata481tcatgcacgaaaagcaaacaaacttgtgtgcttcattggatgttcgtaccaccaaggaat541tactggagttagttgaagcattaggtcccaaaatttgtttactaaaaacacatgtggata601tcttgactgatttttccatggagggcacagttaagccgctaaaggcattatccgccaagt661acaattttttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgc721agtactctgcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtg781tggtgggcccaggtattgttagcggtttgaagcaggcggcagaagaagtaacaaaggaac841ctagaggccttttgatgttagcagaattgtcatgcaagggctccctatctactggagaat901atactaagggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattg961ctcaaagagacatgggtggaagagatgaaggttacgattggttgattatgacacccggtg1021tgggtttagatgacaagggagacgcattgggtcaacagtatagaaccgtggatgatgtgg1081tctctacaggatctgacattattattgttggaagaggactatttgcaaagggaagggatg1141ctaaggtagagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcg1201gccagcaaaactaaaaaactgtattataagtaaatgcatgtatactaaactcacaaatta1261gagcttcaatttaattatatcagttattaccctgcggtgtgaaataccgcacagatgcgt1321aaggagaaaataccgcatcaggaaattgtaaacgttaatattttgttaaaattcgcgtta1381aatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat1441aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtcca1501ctattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggc1561ccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcacta1621aatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtg1681gcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg1741gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcg1801cgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcg1861ctattacgccagctggcgaaggggggatgtgctgcaaggcgattaagttgggtaacgcca1921gggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcacta1981tagggcgaattggagctccaccgcggtggcggccgctctagaactagtggatcccccggg2041ctgcaggaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacc2101cagcttttgttccctttagtgagggttaattccgagcttggcgtaatcatggtcatagct2161gtttcctgtgtgaaattgttatccgctcacaattccacacaacataggagccggaagcat2221aaagtgtaaagcctggggtgcctaatgagtgaggtaactcacattaattgcgttgcgctc2281actgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg2341cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgct2401gcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggtt2461atccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggc2521caggaaccgtaaaaaggccgcgttgctggcgtttttccataggctcggcccccctgacga2581gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata2641ccaggcgttcccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttac2701cggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctg2761taggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccc2821cgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag2881acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt2941aggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagt3001atttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttg3061atccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattac3121gcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca3181gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac3241ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaac3301ttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatt3361tcgttcatccatagttgcctgactgcccgtcgtgtagataactacgatacgggagggctt3421accatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt3481atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc3541cgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaa3601tagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttgg3661tatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgtt3721gtgaaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc3781agtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgt3841aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcg3901gcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaac3961tttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc4021gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttt4081tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg4141aataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaag4201catttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataa4261acaaataggggttccgcgcacatttccccgaaaagtgccacctgggtccttttcatcacg4321tgctataaaaataattataatttaaattttttaatataaatatataaattaaaaatagaa4381agtaaaaaaagaaattaaagaaaaaatagtttttgttttccgaagatgtaaaagactcta4441gggggatcgccaacaaatactaccttttatcttgctcttcctgctctcaggtattaatgc4501cgaattgtttcatcttgtctgtgtagaagaccacacacgaaaatcctgtgattttacatt4561ttacttatcgttaatcgaatgtatatctatttaatctgcttttcttgtctaataaatata4621tatgtaaagtacgctttttgttgaaattttttaaacctttgtttatttttttttcttcat4681tccgtaactcttctaccttctttatttactttctaaaatccaaatacaaaacataaaaat4741aaataaacacagagtaaattcccaaattattccatcattaaaagatacgaggcgcgtgta4801agttacaggcaagcgatccgtcctaagaaaccattattatcatgacattaacctataaaa4861ataggcgtatcacgaggccctttcgtc//
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发布于 ： 2018-03-20
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