Product Name | Somatostatin 14, human, rat, mouse, pig, chicken, frogAGCKNFFWKTFTSC (Disulfide bridge: 3 - 14) |
Size | 1 mg |
Catalog # | AS-24277 |
US$ | $57 |
Purity | % Peak Area By HPLC ≥ 95% |
Somatostatin [SST, GHIH (growth hormone-inhibiting hormone) or SRIF (somatotropin release-inhibiting factor)] is a cyclic peptide existing in two isoforms and is produced in the pancreas islet, GI tract and the central nervous system. Tetradecapeptide Somatostatin-14 (SRIF-14, the originally identified somatostatin) and the 28-amino acid Somatostatin-28 (SRIF-28) have similar biological activities but differ in their potency. Somatostatins inhibit the release of growth hormone in contrast to Growth Hormone Factor (GRF), which stimulates the release of growth hormone. Somatostatins also inhibit the release of prolactin and thyrotropin, peptide hormones from the pituitary gland and glucagon and insulin from the pancreas. It also controls the secretion of gut hormones and functions as a neurotransmitter. | |
Detailed Information | Material Safety Data Sheets (MSDS) |
Storage | -20°C |
References | Kim, D. et al. J Clin Endocrinol Metab 90, 6310 (2005)Meyerhof, W. et al. Proc Natl Acad Sci USA 89, 10267 (1992) |
Molecular Weight | 1637.9 |
AGCKNFFWKTFTSC (Disulfide bridge: 3-14) | |
Sequence(Three-Letter Code) | H - Ala - Gly - Cys - Lys - Asn - Phe - Phe - Trp - Lys - Thr - Phe - Thr - Ser - Cys - OH (Disulfide bridge: 3 - 14) |
Product Citations | McGuire, N. et al. (2013). Gonadotropin releasing hormone (GnRH) and gonadotropin inhibitory hormone (GnIH) in the songbird hippocampus: regional and sex differences in adult zebra finches. Peptides 46, 64. doi: 10.1016/j.peptides.2013.05.007.Keppel, T. et al. (2009). The use of acetone as a substitute for acetonitrile in analysis of peptides by liquid chromatography/electrospray ionization mass spectrometry. Rapid Comm Mass Spectrom 24, 6. doi: 10.1002/rcm.4352.O"Donoghue, A. et al. (2008). Inhibition of a secreted glutamic peptidase prevents growth of the fungus Talaromyces emersonii. J Biol Chem 283, 29186. doi: 10.1074/jbc.M802366200. Zhao, X. et al. (2008). A homogeneous enzyme fragment complementation-based β-arrestin translocation assay for high-throughput screening of G-protein-coupled receptors. J Biomol Screen 13, 737. |
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连续三次都这样,Marker的泳道的位置有很多亮条带,但Marker本来的条带是不亮的。我做了内参和目的,一张胶上左右两部分分别都加样品和Marker,切开转膜分别孵育,一块儿显影,内参的Marker就不会亮,只有目的那一张Marker会亮,昨天做的借了别人的Marker,显影出来还是有那个条带。
我的目的条带还没有Marker那部分的条带亮,Marker的部分非常亮。
湿转,跑胶结束后能看到Marker,转膜条件:300mA,150min,因为目的蛋白160kd左右所以时间比较久.转完之后pvdf膜上却未看到marker,丽春红染色可隐约看到一个条带,请问有哪位高人晓得原因么
已经重复做了4次了,还是曝出9条带。请求各位大神解析解析==
英 [ˈmɑ:kə(r)] 美 [ˈmɑ:rkə(r)]
n.标识,标记; 记号笔,阅卷人; 防守队员; 特征
复数: markers
双语例句
1. Draw your child's outline with a heavy black marker.
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.
步测十英尺,然后在地上做出标记.
英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识,标记; 记号笔,阅卷人; 防守队员; 特征复数: markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.
做western时,看着膜上的Marker条带是直的,但是最后显影出来的目的蛋白的条带全部是歪的,这是为什么?两块胶都是这个问题,欲哭无泪呀!!!求高人指点一下,是什么操作不当导致的
想看下人肿瘤组织内巨噬细胞的浸润情况,我以为一个CD11b就够了。咨询了一下抗体公司,竟然让我订三个抗体,分别是CD11b,CD68和CD163,后两者不是M1和M2的分型吗?
另外查了下CD11b也确实在别的一些免疫细胞有所表达,请教下,有专门的特异性抗体吗?没课题没经费,只能省省省了
请教大家一个问题,不同厂家cIEF的Marker能混合使用吗?另外,如果我对其它厂家pI值为6.0的marker进行cIEF测定,那测得的pI值会等于6.0吗?谢谢!
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