
Description:
Quick-loadPurple50bpDNALadderisapre-mixed,ready-to-loadmolecularweightMarkercontainingonetrackingdye.TrackingdyedoesnotleaveashadowunderUVlight.TheQuick-LoadPurple50bpDNALadderconsistsofaproprietaryplasmids,whicharedigestedtocompletionwithappropriaterestrictionenzymestoyield17bandssuitableforuseasmolecularweightstandardsforagarosegelelectrophoresis.ThisdigestedDNAincludesfragmentsrangingfrom50to1,350basepairs.The200and500bpbandshaveincreasedintensitytoserveasreferencepoints.

Quick-LoadPurple50bpDNALaddervisualizedbyethidiumbromidestainingona3%TBEagarosegel.
Massvaluesarefor1µg/lane.
SuggestedLoad:5–10µl/gellane.
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
Storeat(°C) | Concentration | |
GelLoADIngDye,Purple(6X),noSDS | 25 | 6X |
Notes:
PreparationofDNA:Thedouble-strandedDNAisdigestedtocompletionwithappropriaterestrictionenzymes,phenolextractedandequilibratedinstoragebuffer.Werecommendloading5–10μl(0.5–1μg)ofQuick-LoadPurple50bpDNALadderpergellane.Ingeneral,itisrecommendedtoload1µlpermmofgellane.TheQuick-LoadPurple50bpDNALadderisnotintendedforprecisequantificationofDNAmassbutcanbeusedforapproximatingthemassofDNAincomparablyintensesamplesofsimilarsize.Quick-LoadPurple50bpDNALadderisstableforatleast6monthsat25°C.Forlongtermstorage,storeat4°Cor-20°C.Ifstoredat-20°C,mixwellafterthawing.Ifnotwellmixed,theloadmayfloatoutofthewells.1XGelLoadingDye,Purple,noSDS: 2.5%Ficoll®-40010mMEDTA3.3mMTris-HCl(pH8.0@25°C)0.02%Dye10.001%Dye2ebiomall.com






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想看下人肿瘤组织内巨噬细胞的浸润情况,我以为一个CD11b就够了。咨询了一下抗体公司,竟然让我订三个抗体,分别是CD11b,CD68和CD163,后两者不是M1和M2的分型吗?
另外查了下CD11b也确实在别的一些免疫细胞有所表达,请教下,有专门的特异性抗体吗?没课题没经费,只能省省省了
请教大家一个问题,不同厂家cIEF的Marker能混合使用吗?另外,如果我对其它厂家pI值为6.0的marker进行cIEF测定,那测得的pI值会等于6.0吗?谢谢!
英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识,标记; 记号笔,阅卷人; 防守队员; 特征复数: markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.
做western时,看着膜上的Marker条带是直的,但是最后显影出来的目的蛋白的条带全部是歪的,这是为什么?两块胶都是这个问题,欲哭无泪呀!!!求高人指点一下,是什么操作不当导致的
湿转,跑胶结束后能看到Marker,转膜条件:300mA,150min,因为目的蛋白160kd左右所以时间比较久.转完之后pvdf膜上却未看到marker,丽春红染色可隐约看到一个条带,请问有哪位高人晓得原因么
英 [ˈmɑ:kə(r)] 美 [ˈmɑ:rkə(r)]
n.标识,标记; 记号笔,阅卷人; 防守队员; 特征
复数: markers
双语例句
1. Draw your child's outline with a heavy black marker.
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.
步测十英尺,然后在地上做出标记.


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