ProductHighlights:
NexteraDNALibraryPreparationKitsprovideafastandeasyworkflow,enablingsequencing-readylibrariestobegeneratedinlessthan90minutes,withlessthan15minutesofhands-ontime.SamplespreparedwithNexterakitsarecompatIBLewithallIlluminasequencers.
WithNexteratechnology,DNAissimultaneouslyfragmentedandtaggedwithsequencingadaptersinasinglestep,usingstandardlabequipment.Idealforprecioussamplesavailableinlimitedquantity,theprotocolrequiresonly50ngofDNAinput.
Seealistofautomationvendorswithroboticsystemscompatiblewiththiskit
Specifications:
AssayTime | 90minutes |
Hands-OnTime | 15minutes |
InputQuantity | 50nggenomicDNA(compatiblewithmostlargeDNAgenomes) |
MechanismofAction | EnzymaticFragmentation |
Multiplexing | Upto96availableindexes |
SpeciesCategory | Human,Other,Mammalian,Mouse,Rat,Plant |
SpeciesDetails | CompatiblewithmostlargeDNAgenomes. |
SystemCompatibility | MiSeq,NextSeq550,HiSeq3000,MiniSeq,NextSeq500,HiSeq2500,HiSeq4000 |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants,Insertions-Deletions(indels) |
Method | Whole-GenomeSequencing |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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