ProductHighlights:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitisascalableampliconsequencingsolutionthatdeliverssensitiveandspecificresultsfrombothlow-inputandformalin-fixed,paraffin-embedded(FFPE)DNAsamples.Itoffers:
- Accuratevariantdetectionfromaslowas10ngofinputgenomicDNA(gDNA)andchallengingFFPEsamples
- Fullysupported,optimizedworkflowsolutionincludingsimpledataanalysis
- Automation-friendlyworkflowthatcanbecompletedin6hours,withonly3hoursofhands-ontime
LowDNAInputandFFPECompatibility
TheTruSeqCustomAmpliconLowInputLibraryPrepKitisafullycustomizable,amplicon-basedassayfortargetedresequencingstartingfromaslowas10ngofgenomicDNA(gDNA).Thisscalableassayallowsresearcherstocapturemultipletargetsofinterestsimultaneouslyandsequenceupto1536ampliconsinasinglepool,usingasinglereaction.ThislibraryprepkitofferstheflexibilitytoaccommodateFFPEsamples,suchaspreservedtumortissue.
ConfidentAssayDesign
DesigntargetedTruSeqCustomAmpliconoligonucleotideprobeswithDesignStudio,afree,easy-to-useonlinetoolthatprovidesoptimizedcoverage.
LearnmoreaboutDesignStudio
StartaprojectinDesignStudionow(loginrequired)
AssessFFPESampleQualitybeforeSequencing
TheTruSeqFFPEDNALibraryPrepQCKitusesasimpleqPCRreactiontodetermineDNAqualityandprovideguidanceonsequencingparameters.Thisstepensuresthatonlysamplesthatwillachievethenecessarysequencingmetricsareprepared,conservingresourcesthatmightbeusedonpotentiallylow-quality,unrecoverablesamples.TheresultsoftheQCstepdeterminetherecommendedamountofinputDNA.TheTruSeqFFPEDNALibraryPrepQCKitcanbebundledwiththeTruSeqCustomAmpliconLowInputLibraryPrepKittomaximizelabbudgets.
ForFFPEapplications,wesuggestdesigningyourprojectwith150bpor175bpampliconssinceFFPEDNAishighlydegraded.TruSeqCustomAmpliconLowInputcannotbeusedwithapplicationsrequiring425bpamplicons.
SpeciesCompatibility
TheTruSeqCustomAmpliconLowInputLibraryPrepKitsupportshuman,mouse,rat,andbovinesamples.DesignStudiodesignalgorithmsarealsoavailableformaize,rice,pig,dog,soybean,chicken,andsheep;however,assayperformancecannotbeguaranteedastheresultingassayshavenotbeentested.Inaddition,IlluminaConciergeservicescandesigncustomassaysand/orprovideassayoptimizationsupportforanyspeciesofinterest.
InquireaboutIlluminaConciergeservices
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 4hours |
InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
Multiplexing | Upto384samplespersequencingrun |
MechanismofAction | Amplification |
TruSeqCustomAmpliconLibraryPrepProtocol:
TheTruSeqCustomAmpliconassayisasimpleandstreamlinedmethodforcapturingandamplifyingtargetedregionsofinterest.SeamlessAmpliconSequencingWorkflow:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitispartofanintegrated,fullysupportedworkflowforampliconsequencingthatguidesresearchersfromdesignthroughdataanalysis.Illuminatechnicalandfieldspecialistshelpensurerapidresolutionandminimizepotentiallaboratorydowntime.RobustVariantDetectionatLowDNAInput:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitdemonstrateshighconcordancebetweenexpectedandobservedvariantfrequenciesfor10nghigh-qualityFFPEDNA.ReferenceDNAsampleswerepreparedfollowingtheTruSeqCustomAmpliconLowInputworkflowwiththeTruSeqAmplicon-CancerPanelprimerpool,andsequencedontheMiSeqSysteminreplicatesof4.VariantswerecalledusingMiSeqReporterSoftware.R2valuesareshown.ebiomall.com
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普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败,此外,由于采用了剧烈的玻璃珠击打来破裂菌体,常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA,通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱,可以最大程度的去除这些杂质,同时加上多次柱漂洗,确保得到的DNA具有极高纯度,此外独特的抽提和裂解体系可以迅速裂解细胞(壁)和灭活细胞内核酸酶,不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性。
产品特点:
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。
3.兼容性强,适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物,保证了极高纯度,OD260/OD280典型的比值达1.7~1.9。
5.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
6.快速,简捷,单个样品操作一般可在60分钟内完成。
你找下,杭州昊鑫生物
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
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