ProductHighlights:
- Unlocksmallsamples(50–100ngDNAinput)
- CpG,CHH,&CHGregionsincludedforcomprehensive,whole-genomeresults
- Fastprotocol–five-hourmethod
- Capturefullsamplediversity
Sequencetheentiresample–nolossofinformation
TheprocessofbisulfitetreatmentdenaturesgenomicDNAintosinglestrandedDNA.TruSeqDNAMethylationconvertssinglestrandedDNAintoanIlluminasequencinglibrary.AllssDNAfragmentsarecapturedintoanIlluminasequencinglibrary,thereforeeliminatingthelossofdiversityassociatedwithothermethods.
Supportedanalysisinthecloud
TruSeqDNAMethylationlibrariescanbealignedtothehumangenomeandcomparedfordifferentialmethylationwithIlluminaBaseSpaceAppsMethylSeqandMethylKit.TheseapplicationsarefullysupportedandweredevelopedspecificallyforTruSeqDNAMethylationlibrarypreparation.
ExampledatasetsforTruSeqDNAMethylationlibraries,alsoavailableinBaseSpaceSequenceHubDataCentral(usethe“MethylSeq”categoryfilter),demonstrateunparalleledqualityandseamlessanalysis.
Deepcoverageofcriticalgenomicregions
DepthofcoverageisenhancedingenomicareaswithBIOLOGicalutility.TruSeqDNAMethylationcapturesfullsamplediversityofcriticalareas,including:
- Codingregionstartandendforexonsfromthecanonicaltranscriptofproteincodinggenesforgenesknowntobeinvolvedincancer,takenfromSOMAandCRUKpanels(aswellasliterature-derivedcancergenes)
- GenesdefinedbytheAmericanCollegeofMedicalGeneticsasbeingmedicallyrelevant(ACMGgenes)
- ExoniccodingregionsfromEnsemble70
- Listof100promotersdefinedbytheBroadInstituteasbeingofhighinterestanddifficulttosequence
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根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
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