ProductHighlights:
TruSeqDNAPCR-FreeLibraryPreparationKitsprovidesimple,all-inclusivelibrarypreparationforwhole-genomesequencingapplications.Researcherscansequenceawidevarietyoforganisms,fromsmallgenomessuchasbacteriatohumanwhole-genomes.Thekitsoffer:
- Shortenedgel-freeworkflowsthatremovetheneedforPCR
- ABIlitytosequencethemostchallengingregions
- Improvedgenomecoveragetoidentifythegreatestnumberofvariants
Sequencethemostchallengingregions
TruSeqDNAPCR-freekitsoffersuperiorcoverageofareaswhicharetrADItionallydifficulttosequencesuchashighGC-richregions,promoters,andrepetitivecontent.
ThekitsaretunabletoavarietyofreadlengthsandaresupportedonallIlluminasequencinginstruments.Thispermitstheresearchertotailoreachruntotheneedsoftheexperiment.
Detectthegreatestnumberofvariants
PCR-freemeansreducedlibrarybiasandgaps.Theresultisunsurpasseddataquality.Thisenablesyoutodetectthegreatestnumberofvariants.ExcellentgenomecoveragemeansyourresultshavethelowestandsmallestnumberofgapsandenhancedcoverageofhighG/Crichregions.
UsePCR-freeforfasterprotocols
RemovingPCRcreatesafasterprotocolandsuperiordataquality.Bead-basedsizeselectionshortenstheworkflow.IntandemwithIlluminasequencingsystems,theTruSeqDNAPCR-FreeLibraryPreparationKitprovidesarangeofenhancementstotheindustry"smostwidelyadoptedlibrarypreparationworkflow.
AccessflexIBLethroughputoptions
Kitsincludereagents,samplepurificationbeads,andindexes,withtwooptionsforflexibility:
- TruSeqDNAPCR-FreeLTLibraryPreparationKitssupport24-plexmanualprocessingforlow-throughputstudies.
- TruSeqDNAPCR-FreeHTLibraryPreparationKitsare96-plexforhigh-throughputstudies,andcanbeautomatedonliquidhandlingrobots(orprocessedmanually).
Findanup-to-datelistofautomationvendorswithroboticsystemsthatsupporttheHTlibrarypreparationkits
Specifications:
InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500,NextSeq550,HiSeq3000,HiSeq1000 |
AutomationCapability | LiquidHandlingRobots |
VariantClass | TranscriptVariants,SingleNucleotidePolymorphisms(SNPs),GeneFusions |
SpeciesCategory | Rat,Mouse |
Method | Whole-TranscriptomeSequencing |
Technology | Sequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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