ProductHighlights:
- StudyactivemRNAtranslationwithsequencing
- Sequenceribosome-protectedmRNA
- Predictproteinabundance
- Investigatetranslationalcontrol
- Measuregeneexpression
- Rapid,scalablespin-columnmethod
- Noultracentrifugerequired
- CompatIBLewithyeastandmammaliansamples
Sequenceactivelytranslatedtranscripts
Ribosomeprofilingisatechniqueforinvestigatingtranslationalcontrolthatprovidesasnapshotofalltheribosomesactiveinacellataspecifictime.TruSeqRiboProfilegeneratesRNA-Seqlibrariesfromribosome-protectedfragmentsofmRNAsthatareactivelybeingtranslated.
UseTruSeqRiboProfiletosequencemRNAfragmentsundergoingtranslationbyribosomes.ThesemRNAfragmentsarecalled"footprinted"orribosome-protectedmRNAfragments.
YoucanalsoidentifyproteinsbeingactivelytranslatedfromsamplespreparedwithTruSeqRiboProfile.Samplescollectedatdifferenttimesoftenshowchangesintranslation.Samplestreatedwithdifferentdrugsoftenshowdifferenttranslationpatterns.
Specifications:
InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500 |
AutomationCapABIlity | LiquidHandlingRobots |
VariantClass | TranscriptVariants |
SpeciesCategory | Rat |
ebiomall.com
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根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
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