ProductHighlights:
TruSeqStrandedTotalRNAwithRibo-ZeroGlobinkitsdeliveraclearandcomprehensiveviewofthetranscriptomefromblood-derivedRNA,withafast,efficientsequencinglibrarypreparationworkflow.
High-qualitywhole-transcriptomeanalysiswithprecisestrandinformation
ThesekitscouplethebenefitsofRibo-ZeroribosomalRNAreductionchemistrywithRNAsequencing(RNA-Seq)technologyforwhole-transcriptomeanalysisofhuman,mouse,orratsamples.Thistechnologyprovides:
- Uniformcoverageandprecisemeasurementofstrandorientation
- High-confidencediscoveryofalternativetranscripts,antisenseexpression,andallele-specificexpression
- Discoveryacrossbothcodingandmultipleformsofnon-codingRNA
EfficientribosomalRNAandglobinmRNAremovalinasinglestep
Whole-transcriptomeanalysisofblood-derivedRNArequirestheremovaloftwoformsofabundantRNA—ribosomalRNA(bothcytoplasmicandmitochondrial)aswellasglobinmRNA,whichispresentinhighlevelsinwholeblood.
TrADItionalremovalmethodsrequiretwoindependentsteps.Thismeanstheneedforadditionalreagents,alongerworkflow,andmoreinputRNAlost.TruSeqStrandedTotalRNAwithRibo-ZeroGlobinleveragesRibo-ZerochemistrytoefficientlyremovebothformsofabundantRNAinasingle,rapidstep.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500,NextSeq550,HiSeq3000,HiSeq1000 |
AutomationCapABIlity | LiquidHandlingRobots |
VariantClass | TranscriptVariants,SingleNucleotidePolymorphisms(SNPs),GeneFusions |
SpeciesCategory | Rat,Mouse |
Method | Whole-TranscriptomeSequencing |
Technology | Sequencing |
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根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
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