Description:
KAPADual-IndexedAdapterKit,(15µM)KAPAHyperPlusKits
NGSlibrarypreparation. Evolved.
TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:
- DNAfragmentationandlibraryprepin2.5hours*
- FlexIBLeDNAsampleinputfrom1ng–1µg*
- Reducedbiasandmaximizesequencecoverage*
- PCR-freeworkflows
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
Downloadour AdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Tunableandreproduciblefragmentation
- Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
- ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts
Industryleadinglibraryyields…
- Routinelyachieveconversionrates>80%from≥100nginput*
- SuperiorperformanceacrossarangeofDNAinputamounts*
- HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
…Enablesuperiorsequencingresults
- Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
- Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
Minimalsequencecoveragebias
- Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
- Equivalentperformancetomechanicalshearing*
- Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Applications:
Applications- WholeGenomeSequencing
- WholeExomeSequencing
- TargetedSequencing(custompanels)
- FFPESampleSequencing
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto6monthsat-20ºC.
KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.
Specifications
- SpecDescription
- CompatiblePlatformlluminaHiSeq®,MiSeq®,NextSeq®
- LibraryTypeDNA
- StartingMaterialgenomicDNA,FFPEDNA,PCRamplicons
- InputAmount1ng–1µg
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
暂无品牌问答