Dialysis (using dialysis tubing)
来自 : 蚂蚁淘
Dialysis(usingdialysistubing)
Molecularpourousmembranetubingisusedfordesalting,proteinandDNAisolation/purification.Itcomesinseveraldiametersandporesizes(formolecularweightconversionsfornucleicacidsseegeneralreference).
ThebrandusedinM.SoaresLabisSpectra/PorproducedbySpectrumMedicalIndustries,Inc.(60916TerminalAnnex,LosAngeles,California90060,Phone:(213)650-2100,(800)634-3300),distributedbyFisher.Severalmolecularweightcut-offsanddiametersareavailable.Spectraportubingcomeseitherwet(readytouse)ordry.
Priortouseofdrymembranes:
- Wash30minutesat60°Cinalargevolume(1000x)of2%sodiumbicarbonateand1mMEDTA)
- Rinsetubingthoroughlywithdistilledwater.
- Coolandstoreat4°Cineither1%formaldehyde,1%sodiumbenzoate,or0.1%sodiumazide,deionizedwater,or100%ethanol.Keepthetubingcompletelysubmersed.Uncleanedmembranescanbestoredatroomtemperature.
- Donotautoclave.Heatingover80°Cchangesthemembranecharacteristics(droppermeABIlity).
- TopreventstickingofDNAorchargedproteinstothemembrane,coatwettubinginsideandoutwithasolutionof0.05%BSAin1xTBEbuffer.
- ThepHrangeformembranesis2-12.
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