Description:
UsetheCFXConnectReal-TimePCRDetectionSystemfortwo-targetanalysis.ThispackageincludestheCFXConnectThermalCyclerChassis,CFXConnect96-WellOpticalReactionModule,SYBR®GreenSupermix,96-wellPCRplates,andplateseals.The96-wellreactionmoduleoffersexcellentthermalperformanceanduniformityacrosstheentireblock.Multipledataacquisitionmodes,includingafast-scanoption,providetheflexibilitytomeetallyourreal-timePCRneeds.
FeaturesandBenefits
- Designedforcontrolbyremotepersonalcomputer(upto4CFXSystems)
- Detectionofupto2targetsperwell,plusachanneldedicatedtosingleplexFRET
- IntegratedsolutionforyourSYBR®GreenorEvaGreenandduplexexperiments
- ThermalgrADIentfeaturethatidentifiesoptimalannealingtemperatureinasinglerun
- CFXMaestroSoftwareandqbase+Softwareforreliablevalidation,dataanalysis,andexportofRDMLfilesforconformancewithMIQEguidelines
TheCFXConnectReal-TimePCRDetectionSystemfeaturessixindependentlycontrolledthermalelectricunits,providingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).Theprogrammablethermalgradientfeatureallowsatemperaturegradientofupto24°Cacrossthereactionblockatanystepinaprotocol.
Theopticalsystem,withthreefilteredLEDsandthreefiltered–photodiodes,collectsdatafromallwells,detectinguptotwotargetsperwell.Forsingle-colorFAM,EvaGreen,andSYBRGreenIassays,thefast-scanoptionreadssingle-channelfluorescenceinall96wellsinjust3seconds.AseparatechannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
ProductComponents
- CFXConnectThermalCyclerChassis
- CFXConnect96-WellOpticalReactionModule
- USBcable
- CFXMaestroSoftware
- Instructionmanual
- qbase+Softwarelicense
- SsoAdvanced™UniversalSYBR®GreenSupermix(1725270)
- Hard-Shell®Low-ProfileThin-Wall96-WellSkirtedPCRPlates(HSP9601)
- Microseal®"B"AdhesiveSeals(MSB1001)
ConfigurationOptions
TheCFXConnectReal-TimePCRDetectionSystemcanbeconfiguredwithotherPCRinstrumentssothatitcanbesoftwarecontrolled.InstrumentpermutationsallowuptofourdifferentCFXConnect,CFX96™,orCFX384™SystemstobecontrolledbyonePC.Youcanstartthesameexperimentonallfourinstrumentsatonce,orstartarunononeinstrumentwhileanotherisrunningadifferentprogram.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
ServicePlansandValidationToolsforCFXSystems
- Ensurethatyourreal-timePCRsystemsarefunctioningoptimallywithBio-RadExpertCareService;choosefromseveralserviceplanstokeepyourCFXAutomationSystemIIrunningitsbest
- CertifysysteminstallationandvalidatesystemperformancewithCFXSystemIQ/OQServicesandCFXAutomationSystemIIIQ/OQServices
- VerifytheperformanceofyourCFXSystemswithThermalValidationServicesandtheCFXQualificationPlates
RelatedProducts
- PX1™PCRPlateSealer
- PCRplasticsoptimizedforCFXReal-TimePCRDetectionSystems:
- Hard-Shell96-WellLowProfileSkirtedPCRPlatesincludingbarcodedplates(HSP9955)
- Hard-Shell384-WellSkirtedPCRPlatesincludingbarcodedplates(HSP3905)
- Microseal"B"AdhesiveSeals(MSB1001)
- OpticallyClearHeatSeals(1814030)
- CFXMaestroSoftware,SecurityEdition,forcompliancewithU.S.FDA21CFRPart11regulations SecurityEdition,forcompliancewithU.S.FDA21CFRPart11regulations
- CFXQualificationPlateforperformanceverification,96-well(1845098)
- CFXQualificationPlateforperformanceverification,384-well(1845099)
RelatedCategories
- Real-TimePCRDetectionSystems
- PCRReagentsandqPCRReagents
- PCRPlasticConsumables
- PCRSeals
- PCRAnalysisSoftware
SupportingDocuments
- CFXConnectReal-TimePCRDetectionSystemSpecificationsSheet
- ConnectReal-TimePCRSystemInstallationQuickGuide
- CFXConnect™Real-TimePCRDetectionSystemInstrumentGuideInstructionManual
- Real-TimePCRApplicationsGuide
OnlineResources
- Real-TimePCRDetectionSystemsSelectionChart
- qPCR/Real-TimePCR
- qPCRInstrumentation
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请问PCR仪设置加样量有什么作用,我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系,pcr仪设置时设置了50微升体系会怎么样呢?帮忙回答一下。谢谢~
2、放 PCR 离心管,先把顶盖向上扳,再往前推,露出放样孔,PCR 管
放入前应加好反应体系各组分,再放入 PCR 离心管。
3、反向重复第二步骤将顶盖板向后拉,盖好顶盖。
4、按 F2“Create”新建一个扩增的 PCR 程序。
5、按控制台面右方上下左右方向键,可随意移动编辑位置,输入需要
的温度、时间、循环数等。
6、据 PCR 离心管中加入的反应体积总量,在“Reaction volume”后输入
相应数值,有 Std“1~100ul”和 9600“1~50ul”两档可供选择。
7、按 F1“Start”启动
8、按“INFO”对应键查看 PCR 结束键。
9、扩增完成后按台面左方“Stop”键退出。
10、按“Hist”,可查看上次扩增的历史记录。11、完全退出后,按台面左下方电源开关,拔出插销,切断电源。
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