Vanin-1 Mouse/Rat ELISA Assay
The Vanin-1 Mouse/Rat ELISA Assay is For Research Use Only
Product Developed and Manufactured in Austria by Biomedica
Size: 12×8
Sensitivity: 2.31 pmol/l
Standard Range: 2.31 – 200 pmol/l
Incubation Time: 4.5 hours
Sample Type: Serum, plasma and urine
Sample Size: 5 µl / well
Controls Included
Conversion factor: 1 ng/ml = 19.2 pmol/l (MW: 52.07 kDa)
Assay Principle
The Vanin-1 mouse/rat ELISA Assay is a sandwich enzyme immunoassay for the quantitative determination of mouse/rat Vanin-1 in serum, plasma or urine samples. In a first step, assay buffer is pipetted into the wells of the microtiter strips. Thereafter, standard/control/sample and detection antibody (polyclonal sheep anti-mouse Vanin-1-HRPO) are pipetted into the wells, which are pre-coated with anti-mouse Vanin-1 antibody. Vanin-1 present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In the washing step, all non-specific unbound material is removed. In a next step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzymecatalyzed color change of the substrate is directly proportional to the amount of Vanin-1 present in the sample. This color change is detectable with a standard microplate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) versus standard concentration is generated using the values obtained from the standards. The concentration of Vanin-1 in the sample is determined directly from the dose response curve The kit utilizes recombinant mouse Vanin-1 as a calibrator. The VNN1 gene is conserved in chimpanzee, rhesus monkey, dog, cow, mouse, rat, and chicken.
Related Products
Urine Vanin-1 ELISA Assay
Anti-Beta2 Glycoprotein 1 IgG ELISA
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1；变阻脉冲法，光电比色法
2；荧光干涉法
那种的好一点
以前用countstar,一块计数板5个孔，后来实验室又换了biorad的TC20，一块板子两个孔，只能计两个数，一盒板子30片500块，价格越来越贵。这样我一个12孔板计个数就要花百来块钱啊。大家都是这么用的？板子是塑料的，有没有什么可以清洗之后重复利用的方法？
这样的回答可能没分，好和不好都是比较出来的，一群国产的放在一起比总有个性能比较均衡，比较突出性价比好的，
还是没有和说那个厂家的好桂林的呢好像是优利特这个厂家在做，特康的前身呢就是百特，现在的百特呢基本上是在做试剂，这些东西呢。
也搜索的到的，不过答案还是比较接近的，但想知道的是国产那个厂家生产的比较好，而只回答了一半就和介绍了下国产的血球分析仪有那个几个大的厂家，没有和区分那个最好那个第二那个次点，或许一堆鸡蛋里实在挑不出骨头。
具所知呢迈瑞的好点特康实力没有迈瑞好，迈瑞吸引了一批外资在搞研发，桂林的呢好像有个优利特现在出血分析仪，他做的尿分析仪市场的占有还可以，血的反应不是很强烈。
实在是想找个内行的给比较下。
需要测量转染之后细胞某mRNA表达变化。
实验组给予转染，空白组做对照。
做PCR的时候肯定是要求两组的细胞计数是一样的，请问是转染之前还是转染之后对两组进行计数呢？如果样品两边计数不一样，怎么让做实验的时候两边细胞数目变成一样呢？
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