ProductDescription
ElectrospunGelatinDiscsarefabricatedusingelectrospinningtechniquestocreateaconstructwithauniquestructuresuitableasabiomimeticthree-dimensional(3D)scaffold.Theproductcontainsoverlaidelectrospunfilamentscreatingaporousnetwork,whichpermitscellsandnutrientstoflowcompletelythroughtheporesandprovidesanincreasedsurfaceareaforcellattachment,growthandmigration.
ElectrospunGelatinDiscsarelightlycross-linkedforincreasedmechanicalstrengthanddurABIlityforshortandlong-termtissuecultureexperiments,yetisstillbiodegradableoverthelonger-term.Theaveragefilamentdiametertypicallyrangesfrom500nmto1.5microns.Theelectrospungelatindiscsareapproximately8mmindiameterand1-2mmthick.Theelectrospundiscsfitintoa48wellcultureplateorlarger.Eachpackagecontainsfivediscs.Thisproductissterilizedandready-to-use.
Table1:
Parameter,Testing,andMethod | ElectrospunGelatinDiscs#5214 |
DiscDiameter | 8mm |
PackageSize | 5Discs/Package |
DiscThickness | 0.1-0.2mm |
FiberDiameter | 500nm-1.5um |
StorageTemperature | RoomTemperature |
ShelfLife | Minimumof6monthsfromdateofreceipt |
SterilizationMethod | IrrADIation |
GelatinSource | PorcineGelatin |
DirectionsforUse
DownloadthefullPDFversionorcontinuereadingbelow:
- PreparationandSeeding:
Note:Cellattachmenttothediscisgenerallythemostcriticalstepintissueculture.Temperature,pH,gasexchangeandcellconcentrationcanaffecttherateandefficiencyofattachment.Optimumseedingratedependsonthetypeofcellbeingcultured.
- AsepticallyremovetheElectrospunGelatinDiscsfromthepackaginginalaminarflowworkstation.
- Carefullyplacethediscsintothewellsofa48welltissuecultureplateorlargerusingasterileinstrument.Becarefulnottodamagetheproductasitisbeingtransferred.Itisrecommendedtousenon-treatedtissuecultureplasticware.Note:Tissue-coatedplasticwaremayneedtobecoatedwithagarosetopreventcellattachmenttotheplasticandpromoteattachmenttothedisc.
- SUSPendcellsatdesiredconcentration(approximately5,000cells/cm2)anddispensesufficientvolumeofcellsolutionontopofthediscplacedinthewell.
Note:Avoiddispensingthecellsolutiontoorapidlyasthismaycausedamagetothesponge.
- Transfertoa37ºCincubatorforabout1–2hourstoallowforinitialcellattachment.
- After1–2hour,removetheplatefromtheincubatorandcheckforcellattachment.Additionaltestingmayberequiredtooptimizethetimeittakesforthecellstoattachtothediscs.Checkthemorphologyofthecells.Celladherenceandspreadingwilldictatethetimeneededforattachment.
- Oncethecellshaveadequatelyattachedtothedisc,increasethefinalvolumeineachwelltofullycoverandprovideadequatemediumfortheculturesystem.
- ChangingtheMedia:
- Changethemedia24to36hoursaftertheinitialseeding.Thefrequencyofchangeswillbedeterminedbycelltype,cellattachmentefficiency,andpH.Morefrequentmediumchangesmayberequiredcomparedto2Dculturesystems.
- HarvestingofCells:
Note:Proteasedigestionisthestandardmethodofreleasingcellsfromthediscs.Thestrengthoftheattachmentofthecellstothediscswillvaryfromcelllinetocellline.Theenzymeconcentrationanddigestiontimewillvarydependingupontheactivityoftheenzymeandtheconfluenceofthecells.Collagenaseand/ortrypsinmaybethepreferredmethod.
- WashingthediscswithEDTA-PBSmayassisttheproteasedigestion.Addsufficientvolumetocoverthedisc.
- AspiratetheEDTA-PBSsolutionfromthewell.
- Addsufficientdissociationsolutiontothewelltofullysubmergethediscs.
- Transfertoa37ºCincubator.Checkforcelldetachmentperiodically.
- Oncethecellshavefullydetached,removethecellsanddispenseinacentrifugetube.
- Centrifugethecellsasrequired.
ProductCertificateofAnalysis
SafetyandDocumentation
SafetyDataSheet
CertificateofOrigin
ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
美国AdvancedBioMatrix(简称ABM) www.advancedbiomatrix.comAdvancedBioMatrix(简称ABM)是美国一家著名的生物公司,获得了AllerganInc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺),将Allergan的专业和技术用于蛋白生产与检测,致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。AdvancedBioMatrix不断丰富已有产品线,目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。AdvancedBioMatrix是组织培养,细胞分析和细胞增殖三维(3D)应用的生命科学领域的领导者。我们的产品被公认为纯度,功能性和一致性的标准。我们在生产,分离,纯化,冷冻干燥,细胞培养和蛋白质测试,粘附肽,附着因子,底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。我们的专业技术和知识正在被用来确保我们的产品质量最高,批次之间一致且易于为我们的研究客户使用。
美国AdvancedBioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。AdvancedBioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3Dmatrix 产品开发方面有着丰富的经验。AdvancedBioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。以下为AdvancedBioMatrix3DMatrices 产品竞争优势:1. 提供高纯度和成分确定的胞外基质;2. 超过1000余篇文献引用PureCol产品,品质非常均一;3. 在3D培养基领域可提供最全面的产品线;4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft);5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养);6. 如果客户首次接触3D胶原凝胶,AdvancedBioMatrix还是唯一的预制胶原蛋白(PureColEZGel)供应商;
以下产品为AdvancedBioMatrix全球畅销品:1.PureCol 牛源I型胶原蛋白 3mg/ml#5005-100ML2.Nutragen牛源I型胶原蛋白 6mg/ml#5010-50ML3.FibriCol 牛源I型胶原蛋白 10mg/ml#5133-20ML4.VitroCol 人源I型胶原蛋白 #5007-20ML5. 弹性蛋白原 #5052-1MG6.ECMSelectArraykitUltra-36#5170-1EA7.CytoSoft(刚性可变的基底,AdvancedBioMatrix最新添加产品5190-7EA)8. 人III型胶原蛋白 #5021-10MG9. 人IV型胶原蛋白 #5022-5MG10.SilkFibroin溶液 #5154-20ML11.Fibronectin#5080-5MG12.Vitronectin#5051-0.1MG
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一是紫外灯照射出问题了;
也可能是门没关紧,经常接触又断掉,不稳定;
如果成像仪没问题,那很可能就是电脑上操纵拍照的软件在设置上有了问题,应该有配套的说明书的,可以参考;
实在不行,可以报修的,没必要自己找烦恼。
我想除了PCR方面有些问题外,成像仪的使用和调校我也不是很熟悉,请问各位老师,怎么才能使背景变黑,不至于胶都看得清楚呢?我试过调亮度,GAMMA,但是效果不好.
请教各位,使用成像仪的心得,谢谢了
1.接通电源和照相机电源
2.打开开关,预热30分钟
3.打开软件,文件菜单中gel-doc.xr
4.按live/focus,30s后按autoexpose or manualexpose
5.调iris,zoom ,focus调节图像清晰度,之后按freeze拍照,保存。
BIO-RAD 凝胶成像系统 Universal Hood Ⅱ
仪器性能:
拍摄对象:核酸琼脂糖凝胶电泳
X光胶片
软件功能:获取并处理图像
测定图像光密度
操作步骤:
1. 开启成像系统,电脑电源
2. 打开Quality One软件,调整光源所示图像
3. 观察并调整曝光时间,获得图像并保存
4. 调整图像,并对图像进行光密度分析
5. 关闭所有电源
凝胶成像仪genegeniusbioimage(syngene公司)系统,我实验室是2006年直接进口了这台仪器,时间太久远,连工程师的号码都是空号了,用过这台仪器的师兄师姐也都毕业不知道了找谁了,请问哪位实验室有这个公司产品的,找一下武汉这边的负责人,我们想联系到再找工程师来维修一下,谢谢!
暂无品牌问答