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ALCIAN BLUE TECHNIQUE
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SECTIONS

4micronParaffinwaxsections


SOLUTIONS

  1. AlcianBluepH3.1
    • AlcianBlue1g
    • 0.5%AceticAcid100ml

  2. AlcianBluepH2.5
    • AlcianBlue1g
    • 3%AceticAcid100ml

  3. AlcianBluepH1.0
    • AlcianBlue1g
    • 0.1MHydrochloricAcid100ml

  4. AlcianBluepH0.2
    • AlcianBlue1g
    • 10%SulphuricAcid100ml

  5. 0.1%Nuclearfastred
    • Nuclearfastred0.1g
    • AluminiumSulphate2.5g
    • Distilledwater100ml


METHOD

  1. Takesectionstowater
  2. StaininalcianbluesolutionpH2.5for5minutes
  3. Washwellinwater
  4. Counterstainin0.1%nuclearfastredfor5minutes
  5. Washinwater
  6. Dehydrate,clear.MountsectionsinDPX
RESULTS
  • Mucins-BrightBlue
  • Nuclei-Red


NOTES

  1. TofurtheridentifyspecifictypesofacidmucinsyouneedtousetheotherpHsolutions.

    • Stronglysulphatedmucins-usepH0.2AlcianBlue
    • Weaklysulphatedmucins-usepH2.5orpH1.0AlcianBlue

  2. WhenusinglowpHsolutionsdrainandblotdryinsteadofwashingatstage(3)ofthemethod.

    NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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