ALCIAN BLUE TECHNIQUE
来自 : 蚂蚁淘
SECTIONS
4micronParaffinwaxsections
SOLUTIONS
- AlcianBluepH3.1
- AlcianBlue1g
- 0.5%AceticAcid100ml
- AlcianBluepH2.5
- AlcianBlue1g
- 3%AceticAcid100ml
- AlcianBluepH1.0
- AlcianBlue1g
- 0.1MHydrochloricAcid100ml
- AlcianBluepH0.2
- AlcianBlue1g
- 10%SulphuricAcid100ml
- 0.1%Nuclearfastred
- Nuclearfastred0.1g
- AluminiumSulphate2.5g
- Distilledwater100ml
METHOD
- Takesectionstowater
- StaininalcianbluesolutionpH2.5for5minutes
- Washwellinwater
- Counterstainin0.1%nuclearfastredfor5minutes
- Washinwater
- Dehydrate,clear.MountsectionsinDPX
RESULTS- Mucins-BrightBlue
- Nuclei-Red
NOTES
- TofurtheridentifyspecifictypesofacidmucinsyouneedtousetheotherpHsolutions.
- Stronglysulphatedmucins-usepH0.2AlcianBlue
- Weaklysulphatedmucins-usepH2.5orpH1.0AlcianBlue
- WhenusinglowpHsolutionsdrainandblotdryinsteadofwashingatstage(3)ofthemethod.
NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.
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