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Description
Details
This antibody recognizes the stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-3 expression.
Sample Data
Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents SSEA-3 Antibody and open histogram represents isotype control. A PE-conjugated anti-Rat IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-3 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).
Details
Details
Concentration | 0.5 mg/mL |
---|---|
Species Reactivity | Human, Mouse |
Host | Rat Monoclonal |
Clone | MC-631 |
Isotype | IgM |
Immunogen | 4-8 cell stage mouse embryos |
Formulation | Aqueous buffer, 0.09% sodium azide. |
Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
Recommended Dilutions | Flow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application. |
Alternative Names | Stage-specific embryonic antigen 3 |
References | Brimble, S.N., et al. (2007) The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62. PMID: 17008424Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702Kannagi, R., et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361. PMID: 6141938 |
Technical Documents | ST11014 Technical Data Sheet |
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而且,紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
不胜感激!!!!
祝大家研究顺利!!!
下面是网上的操作规程
1.超净工作台操作使用前的准备工作
(1)开启超净工作台风机,净化台内环境。打开工作灯达到工作照度。
(2)检查超净工台中准备的各种灭菌饲养和实验物品是否齐全。若需更换笼盒。检查灭菌笼盒准备情况,并用不锈钢推车移至超净台移门边。
(3)检查喷雾器喷洒效果及超净台内外擦抹用的药液是否已配置好。
(4)打开房间内排风系统。排除打开笼盒时逸出的有味气体。
(5)检查IVC机组各种表头指针及目视各笼盒内的动物情况,估测IVC运行情况。
(6)记录影响实验动物繁殖、生长、发育的主要环境参数,如温度、相对湿度、日温差、笼盒内外压差等。
2.超净工作台操作使用程序及规范
(1)人员带好一次性口罩、帽子及医用乳胶手套。
(2)用双手轻轻抬起IVC笼盒外端,沿笼架搁挡向外移出笼盒,放在超净工作台旁的不锈钢小推车上。
(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面,消毒灭菌并沾粘住表面的尘埃,防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中,捞起并拧干药液毛巾,擦干手套外液滴,同时擦一下笼盒的外表。
(4)打开超净工作台移门,把笼盒移入超净工作台。
(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。
谢谢采纳
【消过毒的无菌棉球】分几种
1、放在铁盒子里一起消过毒的
2、放在超净台上、用紫外照过30min的
急求,谢谢了!
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