Product Name | Calcitonin, salmonCSNLSTCVLGKLSQELHKLQTYPRTNTGSGTP - NH2 (Disulfide bridge: 1 - 7) |
Size | 5 mg |
Catalog # | AS-20678 |
US$ | $494 |
Purity | % Peak Area By HPLC ≥ 95% |
A 32-amino acid long peptide with a disulfide bridge between Cys1 and Cys7 and C-terminal amidated Proline, Calcitonin (CT) is involved plasma calcium level. Compared to human or rat calcitonin, Salmon Calcitonin (sCT) is more potent in its biological actions such as inhibition of osteoclasts resorption of bones, renal ion excretion modulation, and others. The reason for its potency has been attributed to the fact that sCT forms an amphipathic helix in its amino acids 9-19 region. | |
Detailed Information | Material Safety Data Sheets (MSDS) |
Storage | -20°C |
References | Andreotti, G. et al. J Biol Chem 281, 24193 (2006), doi: 10.1074/jbc.M603528200 |
Molecular Weight | 3431.9 |
CSNLSTCVLGKLSQELHKLQTYPRTNTGSGTP-NH2 (Disulfide bridge: 1-7) | |
Sequence(Three-Letter Code) | H - Cys - Ser - Asn - Leu - Ser - Thr - Cys - Val - Leu - Gly - Lys - Leu - Ser - Gln - Glu - Leu - His - Lys - Leu - Gln - Thr - Tyr - Pro - Arg - Thr - Asn - Thr - Gly - Ser - Gly - Thr - Pro - NH2 (Disulfide bridge: 1 - 7) |
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这些染料都非常成熟,光毒和淬灭都很低,当然要考虑到你采集图像时的显微镜参数。
比如calcein,常用的示踪,绿光(虽然这些颜色只是根据光谱加上去的伪彩),但要考虑你的实验过程中结合细胞结构,是否会伴随calcein的泄漏,就是荧光降低。
dri,还可以看膜啊
cfda也不错
bcef虽是ph指示,但你试验时不仅可观察细胞,还可看细胞ph变化,也行。
当然你或许还要结合其他方法,如细胞免疫化学等手段去双染或多染,都需要综合考虑染料之间特性。单独染一个染料,有点浪费,不如多染,数据和图像也好看些。现在流行细胞成像。
想请问一下,DAPI这个染料到底有没有膜通透性,我通过百度搜索查询关于DAPI染料的,基本上是说它能透过细胞膜对活细胞和死细胞均能染上蓝色;但是也有人说DAPI只可以透过死细胞膜,不能对活细胞进行染色,用以区分活死细胞,到底哪个是对的啊,蒙了!!!!!!
For principle,look at this site:
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