Introduction Milkfatmeasurementisacommontaskinthedairyindustry,becausemilkfatcontentisonefactorthatdeterminesmilkpriceandisnecessarytoknowforcasein/fatrationormalization.Itisalsoimportantforthedairymantoknowmilkfatcontentexactly:discrepanciesintheresultsofmilkfattests(usuallyperformedinthedairyindustrialplant)haveeconomicrelevance.Inaddition,alowmilkfatcontentcouldindicatetheexistenceofanimalhealthdeficiencies. Principle Thefatcanbeseparatedfromfat-containingmilk/milkpowderthroughtheadditionofsulphuricacid.Theseparationismadebyusingamylalcoholandcentrifugation.Thefatcontentisreaddirectlyonaspecialcalibratedbutyrometeraftercentrifugation. Apparatus Reagents Procedure Hazards Sulphuricacidistoxic,highlycorrosiveandwillcausesevereburningifitcomesincontactwiththeskinoreyes.Whenmixingthebutyrometercontents,considerableheatisgenerated. Ifthestopperisslightlyloose,leakagemayoccurduringmixing,centrifugingorholdinginthewaterbath. Precautions WearprotectiveeyegogglesAvoidallspillageanddroppingofsulphuricacidfromaciddispensers.Whenmixing,holdthebutyrometerstopperfirmlytoensurethatitcannotslip.Useaclothorglovetoprotectthehandswhenmixing.Donotpointthebutyrometeratanyonewhenmixing. Authors**SanjeevSharma1,BRYadav1,AartiBhardwaj2,ShaliniJain3andHariomYadav31LivestockGenomeAnalysisLab,3AnimalBiochemistryDivision,NationalDairyResearchInstitute,Karnal-132001,India2MeerutInstituteofEngeeneringandTechnology,Meerut,U.P.,India