Agrisera/Goat antiChicken IgY (H&L), HRP conjugated/AS09 603/_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall -蚂蚁淘商城

商品列表技术文章相关问答

当前位置： > 首页 > 技术文章 >

Agrisera/Goat antiChicken IgY (H&L), HRP conjugated/AS09 603/

来自 : mayitao


productinformation
Background		Goatanti-chickenIgY(H&L)isasecondaryantibodyconjugatedtoHRP(horseradishperoxidase)whichbindstochickenIgYinimmunologicalassays.
Immunogen		PurifiedchickenIgY,wholemolecule.
Host		Goat
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedgoatIgG,usingsolidphasechickenIgY.
Format		Lyophilized
Quantity		1mg
Reconstitution		Forreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.
Storage		Storelyophilizedmaterialat2-8°C. Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity. Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplications		ELISA(ELISA),Immunohistochemistry(IHC),Westernblot(WB)
Relatedproducts		AS101489\|Rabbitanti-ChickenIgY(H&L),HRPconjugated AS09606\|Goatanti-henIgY(H&L)APconjugated AS09610\|Goatanti-henIgY(H&L)biotinconjugated
Additionalinformation		ThisHRP-conjugateissuppliedin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree0.1%(v/v)ofKathonCGisusedaspreservative.

applicationinformation
Recommendeddilution		1:10000-1:150000(ELISA),1:500-1:5000(IHC),1:20000withenhancedECLand1:10000withstandardECL(WB)
Expected\|apparentMW
Confirmedreactivity		ChickenIgYheavyandlightchains(H&L).
Predictedreactivity
Notreactivein		noconfirmedexceptionsfrompredictedreactivityknowninthemoment
Additionalinformation		Antibodybindsto: heavychainsonchickenIgY lightchainsonallchickenimmunoglobulins Noreactivityisobservedtonon-immunoglobulinchickenserumproteinsbasedinimmunoelectrophoresis. ChickenimmunoglobulinisoftencalledhenorchickenIgG,howeveritisderivedfromeggyolk,thereforeIgY.
Selectedreferences		Huangetal.(2015).EffectsofexogenoussalicylicacidonthephysiologicalcharacteristicsofDendrobiumofficinaleunderchillingstress.PlantGrowthRegulationpp1-10.

applicationexample

5µgoftotalextractfrom(1)Hordeumvulgare totalleaf, (2)Zeamays (3)SpinaciaoleraceaextractedwithPEB(AS08300)wereseparatedon4-12%NuPage(Invitrogen)LDS-PAGEandblotted1htoPVDF.Blotswereblockedimmediatelyfollowingtransferin2%ECLAdvanceblockingreagent(GEHealthcare)in20mMTris,137mMsodiumchloridepH7.6with0.1%(v/v)Tween-20(TBS-T)for1hatroomtemperaturewithagitation.Blotswereincubatedintheprimaryanti-PsbAantibody (AS01016)atadilutionof1:10000for1hatroomtemperaturewithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashedoncefor15minand3timesfor5mininTBS-Tatroomtemperaturewithagitation.Blotswereincubatedinsecondaryantibody(anti-henIgGhorseradishperoxidaseconjugated,AGRISERA)dilutedto1:50000in2%ECLAdvanceblockingsolutionfor1hatroomtemperaturewithagitation.Theblotswerewashedasaboveanddevelopedfor5minwithECLAdvancedetectionreagentaccordingtothemanufacturersinstructions.ImagesoftheblotswereobtainedusingaCCDimager(FluorSMax,Bio-Rad)andQuantityOnesoftware(Bio-Rad).Exposuretimewas30seconds.