productdatasheet
CatalogNo.:56464(cloneS127-31)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
TheSAPAPproteinfamilyhasatleastfourmembersandundergoescomplexalternativesplicing.Theyarethoughttobeadaptorproteinsthatinteractwithdifferentsynapticscaffoldingproteins,cytoskeletal,andsignalingcomponents.SAPAPinteractswithGKdomainsofPSD-95andalsobindsShankattheC-terminalregion.SAPAPsappeartobeimportantinrecruitingandclusteringofNMDAreceptorsandMAGUK(Membrane-AssociatedGuanylateKinase)-basedsignalingcomplexes.
Thisantibodyrecognizeshuman,mouseandratSAPAP1,2,3,and4.
APPLICATIONSImmunoblotting:useat1-10ug/ml.Bandsof~120kDa(SAPAP2)and~110kDa(SAPAP1/3/4)aredetected.
MBM=mousebrain,RBM=ratbrain,
1stHBM=humanhippocampus,2ndHBM=humancerebralcortex.
ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Ratbrainlysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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