Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.
Symbols/Related Terms:
- COL1A1
- COL1A2
- COL3A1
- Collagen type III, alpha 1
- osteogenesis imperfecta
ebiomall.com
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E.coliExpressionSystems.pdf(136.05k)
或者可以过柱纯化,过柱纯化要加一半的佐剂,所以纯化后浓度要高于500ug/ml为宜,否则要增加免疫的次数。
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