TwotypesofsolubleTNFreceptors(sTNFR-1andsTNFR-2)havebeenidentified,whichacttoneutralizetheBIOLOGicalactivitiesofTNF-alphaandTNF-beta.Levelsofthesesolublereceptorsappeartoariseasaresultofsheddingoftheextracellulardomainsofthemembraneboundreceptors.ElevatedlevelsofsolubleTNFreceptorshavebeenfoundintheamnioticfluidofpregnantwomen.
TherecombinantsTNFR-2comprisesa184aminoacidfragment(23-206)correspondingtothematuresTNFR-2proteinandisexpressedinE.coliwithanamino-terminalhexahistidinetag.
Form:liquid
Purity:95%SDS-PAGE
Storage:Storeat-20°C.
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E.coliExpressionSystems.pdf(136.05k)
或者可以过柱纯化,过柱纯化要加一半的佐剂,所以纯化后浓度要高于500ug/ml为宜,否则要增加免疫的次数。
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