Assay Format
- Human
- Hydrothorax
- Plasma
- Serum
- Semi-Quantitative
Product Specifications
Product Feature
- Small sample volume required (2 µl)
- Detects antibody specificity
- Detects autoantibody expression
- Affordable, quick and simple to use
Target Name
Scroll over each spotted protein for more information | ||||
14-3-3 theta
| Annexin A1
| Annexin A2
| CAGE
| Cathepsin D
|
CDK2
| c-Myc(MYC)
| Cyclin A
| Cyclin B1
| Cyclin D1
|
DKK-1
| GBU4-5
| HSP70-9B/HSPA9/GRP-75
| Hu-D
| IMDH2
|
IMP1
| IMP2/IGF2BP2/p62
| IMP3
| LAMR1
| MAGEA4
|
MALAT1
| NY-ESO-1
| p53
| Paxillin
| PGAM1
|
PGP9.5
| Recoverin
| SOX2
| Survivin(BIRC5)
| Ubiquilin-1
|
Application Notes
- RayBio® Human Lung Cancer IgG Autoantibody G1 Array Slide
- RayBio® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
- Blocking Buffer (8 ml/bottle)
- Wash Buffer 1 (30 ml/bottle)
- Wash Buffer 2 (30 ml/bottle)
- 1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)
- 1,500X Streptavidin-Conjugated Fluor (1 µl/vial)
- Small plastic boxes or containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- Distilled water
- Aluminum foil
- Laser scanner for fluorescence detection
- See Manual for relevant protocols.
Typical Data
The following image is a typical result of the Human Lung Cancer Autoantibody Protein Array. The arrays were incubated with 1:200 diluted serum samples. The results shown in Fig. A indicate no background from the biotin-labeled anti-human IgG antibody or from the fluorescent dye-labeled streptavidin. In Fig. B, a base background level is established from normal serum. This background can be subtracted during data analysis without affecting the results. Lastly, Figures C and D are the results of two individuals diagnosed with lung cancer. Several autoantibodies show clear binding.
Storage/Stability
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