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ubpbio/Z-Leu-Leu-Glu-AMC (Z-LLE-AMC)/G2100/5 mg
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4000-520-616
Z-LLE-AMCisafluorogenicsubstrateforthepeptidylglutamylpeptidehydrolyzing(PGPH)activityorthecaspase-likeactivityofthe20Sand26Sproteasomes.Workingconcentrationsofthissubstrateis50-200µM.ThereleasedAMCcanbedetectedbyafluorimeterorplatereaderatexcitation/emissionwavelengthsof380nm/460nm,respectively.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
AdditionalInformation
ProductName: | Z-Leu-Leu-Glu-AMC(Z-LLE-AMC) |
---|---|
AlsoKnownAs: | Z-LLE-AMC |
CatalogNo.: | G2100 |
Size: | 5mg |
MolecularWeight: | 664.8Da |
Species: | N/A |
Source: | Synthetic |
Stock: | Powder |
Concentration: | N/A |
QualityAssurance: | >98%byHPLCandNMR |
Storage: | EligIBLeforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO. |
PDFDataSheet: | DownloadPDFdatasheet |
NCBIRefSeq: | N/A |
Image(s): | No |
ShippingMethod: | Roomtemperatureshipping |
References: | 1.ChenP,etal.(1996)Cell86(6),961–972. 2.ArendtCS,etal.(1997)ProcNatlAcadSci94(14),7156–7161.3.ReidlingerJ,etal.(1997)JBiolChem272(40),24899–24905. |