ElectroelutionofagarosefragmentsElectroelutionbuffer1MTris,pH7.512.0mls0.5MEDTA0.24mls1MNaCl3.0mlsqsto600mlsdH2OAcetatecushion3MNaAcetatepH4.8480ul0.1%BromphenolBlue40ul1.Placegelslicesintrough2.Removeallairbubbles,thenlayer80ulofacetatecushion3.Electroeluteat:120Vfor~1Kbto140Vfor>2.5Kbfor40minfor~1Kbto60minfor>2.5Kb4.Collect~300ulofsaltcushion,add3Xvolumesof95%ethanoltoprecipitate5.RemovegelslicesCleanwellsRunfor10minlongerCleanwellsagainRinsethoroughtlytoremoveanyextraneousDNA