Concentration5u/μl 5 " . . . A↓C C G G T . . . 3 "3 " . . . T G G C C↑A . . . 5 "
Storage Buffer10mM Tris-HCl (pH 7.5), 250mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol.
Unit Definition5u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.
Thermal Inactivation65˚C for 20 minutes.
Quality Control AssaysLigation/ Recutting AssayAfter 5-fold overdigestion with AsiG I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AsiG I for 16 hours at 37°C.
Ordering Information
Catalog No | Description | Pack Size |
FDRE1126 | AsiG I (Age I) | 25 preps |
DownloadsManualAsiG I (Age I)
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我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根
1970科家致癌RNA病毒发现种特殊RNA病毒聚合酶,该酶能RNA模板,根据碱基互补原则.程与般病毒转录向相反,故称逆转录,催化程酶称逆转录酶,发现哺乳物胚胎细胞裂淋巴细胞含.
逆转录病毒称携带逆转录酶病毒,先侵入宿主细胞病毒RNA模板,靠酶形DNA环化,合宿主细胞染色体原病毒形式宿主细胞代代传.
HIV(艾滋病病毒)典型逆转录病毒
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