Description:
ThelatestdevelopmentoftheflashBACtechnoloy,flashBACULTRA™hastakentosystemastepfurther,bytheremovalofthreemorevirusgenes(p10,p74andp26)fromtheflashBACULTRA™genome.Thishasallowedfurtherimprovementsinrecombinantproteinyieldandqualitytobedelivered.
Deletionofp10increasespolhactivityprovidingmorerecombinantprotein,increasesnuclearandcellularstABIlity,ensuringalongertimeframeforproteinexpressionandremovesamajorcompetitorforlimitingcellularresources.p74isnon-essentialincellculturebutisessentialfororalinfectivityofocclusion-derivedvirus(ODV)inthehostwhereitplaysaroleinmidgutattachmentandfusion.Deletionofp74hasbeenshowntohavenoeffectonvirusproductioninvitro.Deletionofp74furtherincreasesthebiosafetyprofileofrecombinantbaculovirusesintheenvironment,makingthemunabletotraversetheinsectgutwall.P26isanearlygenethatcodesfora240-aminoacidpolypeptideofunknownfunctionandhasthesame5′terminusasp10.Deletionofthe3’-endofp26andfusiontolacZorp10havepreviouslybeenshowntohavenoeffectonvirusreplicationinvitro.Deletionofp10,p74andp26removesanunnecessarygeneticburdenfromtherecombinantvirusgenome,providingamoreefficientbaculovirusexpressionvector.
TakentogetherwiththechitinaseandcathepsindeletionsthismakesflashBACULTRAthebestchoiceforthemostdifficulttoexpressproteins.HoweverflashBACULTRAissuitablefortheexpressionofcytoplasmic,nucleus,secretedandmembraneproteins.
AdditionalInformation:
Name | flashBACUltra-BaculovirusExpressionSystem(24reactions) |
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RelatedProductNames | flashBACUltra-BaculovirusExpressionSystem(24reactions) |
IntendedUse | ResearchUseOnly |