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2011年春季新品特价促销——哺乳动物细胞转染试剂盒

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POLOdeliverer™3000TransfectionReagent

PRODUCTDESCRIPTION

POLOdeliverer™3000TransfectionReagentisasuperiorpolymer-basedformulationforthetransfectionofDNAintoeukaryoticcellsandprovidesthefollowingadvantages:

l Highesttransfectionefficiencyinmanycelltypesandformats

l Simpleprocedurewithoutmediumchange

l Transientandstabletransfectionofadherentandsuspensioncells

l Verylowcytotoxicity

POLOdeliverer™3000givessuperiortransfectionefficiencyforabroadrangeofcelllines,includingandnotlimitedtothefollowingcelllines:

293 CHO-S(adherent) Hela HT29

293A COS-1(w/oserum) PC12 NIH3T3

293T COS7-L MDCK MCF7

PrimaryHumanFibroblasts(w/oserum) Vero HepG2

SHIPPINGCONDITIONS

POLOdeliverer™3000TransfectionReagentisshippedatroomtemperature.

STORAGECONDITIONS

POLOdeliverer™3000TransfectionReagentshouldbestoredat4ºC.

STABILITY

ThestabilityofPOLOdeliverer™3000rransfectionreagentisguaranteedfor6monthswhenithasbeenstoredasrecommended.POLOdeliverer™3000Reagentshouldnotbefrozen.

GeneralprotocolofplasmidDNAtransfectionwithPOLOdeliverer™3000

1. Onedaybeforetransfection,plate3×10⁵cellsin2mlmediumperwellofa6-wellcultureplate.Incubateat37ºC(5%CO₂)overnight.Theconfluencyofcellsshouldbe60-80%whentransfection.

2. Dilute2μgDNAin100μlmedium,suchasDMEM,RPMI1640,opti-MEM.

3. Dilute4μlPOLOdeliverer™3000in100μlthesamemedium.

4. AddPOLOdeliverer™3000solutiontotheDNAsolutiondropwiseandmixbypipetteorvortex,thenincubatedatroomtemperaturefor15minutes(37ºCincubationfor5minutesofthePOLOdeliverer™3000solutionandtheDNAsolutionseparatelybeforemixingisrecommended).

5. Withoutchangingtheculturemedium,addthemixturedropbydropintothecultureandmixbygentlyrockingtheplate.

6. Maintainthetransfectedcellsat37ºC,5%CO₂for24-48hourswithoutmediumchange(Ifyouwant,themediumcanbechanged12–24hoursaftertransfection.).

TableRecommendedconditionsfortransfectionwithPOLOdeliverer™3000*

Culturevessel
96-well
24-well
12-well
6-well
35-mm
60-mm
100-mm
150-mm
T25
T75
Surfacearea(cm²)
0.3
2
4
10
10
20
60
140
25
75
RecommendedvolumeofPOLOdeliverer™3000(μl)
0.4
2
3
4
4
10
20
50
12
40
RecommendedqualityofDNA(μg)
0.2
1
1.5
2
2
5
10
25
6
20
*Fortransfectionindifferentcelllines,optimizationofPOLOdeliverer™3000amount,DNAconcentration,cellnumberandtransfectiontimeisrecommended.

Productnameandcatalognumbers

Name
Size
Catalognumber
POLOdeliverer™3000Reagent
0.75ml
P30001
POLOdeliverer™3000Reagent
1.5ml
P30002
POLOdeliverer™3000Reagent
1.5ml*10
P30003

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