TheProteaseinhibitorcocktailisusedas100Xforextractprepsandas1000Xforcolumnelutionbuffers**.Cocktailismadein100%EtOHandcontains: inhbitor BenzamidineHCL Phenanthroline Aprotinin* PepstatinA* Leupeptin* **somecomponentsarenotcompletelysolublein100%EtOH.VortexsolutionwellbeforeusetoobtainacloudysUSPensionandaddtobuffer-theybecomesolubleinaqueous PMSFisveryunstableinaqueoussolutions.The100mMstocksolutionismadeinisopropanolandstoredinthedarkat-20degrees.Mustbewarmed(37degrees)togetintosolution.Useat1mMinsolutions. inhbitor PMSF
Proteaseinhibitorsarerapidlyinactivatedinaqueoussolutionsandshouldbeaddedtosolutionsimmediatelybeforeuse.Cocktailsolutionsarestoredat-20degreesandaregoodforapproximately6months.
