GenCatch TM PCR Cleanup Kit
Catalog# | size | Price | Quantity | |
---|---|---|---|---|
2360050 | 50 Preps | $45.00 | ||
2360250 | 250 Preps | $185.00 |
Description | GenCatch TM PCR Cleanup Kit purifies DNA molecules from PCR and other enzymatic reactions from enzymes, dNTPs, salts, and primers without phenol/chloroform extraction. Recovery efficiency of DNA fragments ranging from 70 bp to 10 kb in size is up to95%. It totally eliminate slurry carryover problem as encountered using silica beadsor glass milk based methods. Up to 10 ¦Ìg DNA can be recovered with a single columnand elution volumes can be as small as 10 ¦Ìl. | ||||
Detailed Components | High capacity/quality columns with buffers and protocols. | ||||
Features | Capacity: Up to 10 ¦Ìg of DNA. Elution Volume: 10-50 ¦Ìl . Recover Efficiency:Up to 95%. Quality: DNA purifiedusing GenCatch TM PCR Cleanup Kit is free from enzyme, polysaccharides, agraose,carry-over slurry and salt contamination. And is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformationand transfection, radioactive and fluorescent sequencing. Fast: Single samplescan be prepared within 10 minutes with minimum efforts. 18-24 samples canbe simultaneously processed in a centrifugation format within 30 minutes. Or, morecan be processed using a vacuum manifold. Flexible: Mini columnscan used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenolextractions or ethanol precipitations required. | ||||
Technical Data | PCR products of various MW (lane 1-5) were purified using GenCatch TM PCRCleanup Kit and loaded as a cocktail (lane 6) onto 1.5% agraose gel. Recovery efficiencycan be roughly estimated by comparing the overall intensity of the individual bands. |
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比如,你的流动相比例、水相pH值、柱温等等条件稍有变化,色谱峰就对不上了。比如,一个碱性物质检测,流动相的pH值稍有波动,这个碱性物质的保留时间就前后波动的很厉害。这个时候就需要定位。
标准品:一般来说,标准品的结构是确定的,含量是确定的。结构就不用说了,保留时间一致就代表是这个东西了。含量,用外标的话就可以计算出样品的含量了。
这个定位品……我倒是没接触过定位品,就理解成用来定位的物质吧。标准品价格比较贵,自制的标准品通过结构确证可以用来定位。这样确保这个位置的色谱峰是这个物质。
Reference material, accompanied by a certificate, one or more of whose property values are certified by a procedure, which established its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence. (Brammer StandardBASARMIetc). (ISO Guide 30-1992)
标准品CRM:标准物质/标准品附带析证书属性值某项或若干项都按照某程序进行验证验证程序建立些属性值所表述体系精确体现追溯性每经验证数值都伴随着某特定置信水平确定度(BS,NISTBASARMI等)(ISO Guide 30-1992)
Reference Materials(RM)
Materials or substance one or more of whose property values are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning values to materials. (ISO Guide 30-1992)
照品RM:种原料或物质其某种或几种属性值表现充均性并且用于设备校准析评估或者物质定植(ISO Guide 30-1992)
各们前辈,初涉诊断行业,开发PCR诊断试剂,求问微生物标准品或者质控血清(比如支原体、衣原体、金葡菌等)应该到哪里购买啊?中检所和卫生部临检中心好像都没有。谢谢!
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