人RPS18内参引物,10μM
概述
实时荧光定量PCR(定量PCR)技术能够非常灵敏的对核酸进行定量。在研究不同细胞类型,发育阶段,和/或样品处理时,内源性参照基因的选择就至关重要。根据GenBank上公布的序列,通过BLAST搜索和实验验证,推出了高特异性的12对人内源性参照基因的引物。使用此内参引物可以获得更为准确的靶基因表达结果
特点
- 扩增长度:50-200bp;
- 引物长度:18-23核苷酸;
- GC含量:40-60%;
- 退火温度:60°c
- 特异性:全部引物均通过与全mRNARefSeq数据库BLAST比对设计并经实验验证。
应用
荧光定量PCR内参
人内源性参照基因引物产品编号:B661101_B661102_B662102_B661103_B661104_B662104_B661105_B661106_B661107_B661108_B661109_B661110_B661111_B661112包装规格:100µl组分编号名称浓度规格B661101HS-RPS1810µM100µlB661102HS-ACTB10µM100µlB662102HS-ACTB100µM100µlB661103HS-B2M10µM100µlB661104HS-GAPDH10µM100µlB662104HS-GAPDH100µM100µlB661105HS-PPIA10µM100µlB661106HS-HPRT110µM100µlB661107HS-YWHAZ10µM100µlB661108HS-TBP10µM100µlB661109HS-UBC10µM100µlB661110HS-RPLP010µM100µlB661111HS-TFRC10µM100µlB661112HS-RPL3210µM100µl产品介绍实时荧光定量PCR(定量PCR)技术能够非常灵敏的对核酸进行定量。在研究不同细胞类型,发育阶段和/或样品处理时,内源性参照基因的选择就至关重要。生工根据GenBank上公布的序列,设计了高特异性的12对人内源性参照基因的引物。通过BLAST搜索和实验验证,证明了全部引物序列的基因特异性,结果表明引物结合位点无多位点匹配的现象。使用此内参引物可以获得更为准确的靶基因表达结果。产品特点扩增长度50~200bp引物长度18~23个核苷酸GC含量40~60%退火温度60°C特异性BLAST比对,实验验证SangonBiotech引物列表基因名扩增长度(bp)位置NCBIAccessionNo.全称Rps18106exton2/3NM_02255118SRibosomalRNAACTB144exton5/6NM_001101β-actinB2M114exton2/3NM_004048β-2-microglobulinGAPDH138Exton6/7NM_002046Glyceraldehyde-3-phosphatedehydrogenasePPIA97exton1/3NM_021130PeptidylprolylisomeraseAHPRT1131exton1/2NM_000194Hypoxanthinephosphoribosyltransferase1YWHAZ103exton2/3NM_145690Tyrosine3-monooxygenaseTBP132exton5/6NM_003194TATAbindingproteinUBC123exton1/2NM_021009UbiquitinCRPLP0101exton2/3NM_001002RibosomalproteinlargeP0TFRC156exton2/3NM_003234Transferrinreceptor(p90,CD71)RPL32100exton3/4NM_000994RibosomalproteinL32相关产品编号名称规格B661201RatEndogenousReferenceGenesPrimers100µlB661301MouseEndogenousReferenceGenesPrimers100µlB532957SGExcelUltraSYBRMixture5mlB532957SGExcelUltraSYBRMixture1mlB532956SGExcelUltraSYBRMixture(WithROX)5mlB532956SGExcelUltraSYBRMixture(WithROX)1mlB532955SGExcelFastSYBRMixture5mlB532955SGExcelFastSYBRMixture1mlB532954SGExcelFastSYBRMixture(WithROX)5mlB532954SGExcelFastSYBRMixture(WithROX)1mlB630001Green-2-GoqPCRMastermix4x1.25mlB630002Green-2-GoqPCRMastermix4x1.25mlB630003Green-2-GoqPCRMastermix4x1.25mlB630004Green-2-GoqPCRMastermix4x1.25ml