Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | Epinephrine-Norepinephrine ELISA kit - Research |
Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only. |
Format | 3 x 96-well plate |
Samples | Any sample |
Sample volume | 1 - 750 µl |
Cross specie-reactivity | React with all species |
Standard range | Epinephrine, Dopamine: 0 / 0.5 - 80 ng/ml; Norepinephrine: 0 / 0.2 - 32 ng/ml |
Sensitivity | Epinephrine, Dopamine: 10 pg/ml Norepinephrine: 4 pg/ml |
Specificity | No significant cross-reactivity was observed with Epinephrine, Norepinephrine and Dopamine analogs such as Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
Assay time | Sample preparation 4 hours and ELISA overnight |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
ELISA | Dopamine, Epinephrine, Norepinephrine antisera overnight incubation, revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
- Horckmans M et al. Gene deletion of P2Y4 receptor lowers exercise capacity and reduces myocardial hypertrophy with swimming exercise. Am J Physiol Heart Circ Physiol. 2012 Oct 1;303(7):H835-43.
- Schneeberger M et al. Deletion of miRNA processing enzyme Dicer in POMC-expressing cells leads to pituitary dysfunction, neurodegeneration and development of obesity. Molecular Metabolism. 2013 Apr.
- Ohlsson C et al. Estrogen receptor-α expression in neuronal cells affects bone mass. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):983-8.
- Vida G et al. α7-cholinergic receptor mediates vagal induction of splenic norepinephrine. J Immunol. 2011 Apr 1;186(7):4340-6.
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各位老师,在用凝集法或去溶剂法制备BSA的纳米微球,查阅相关文献,使用100mg的BSA溶于10ml的去离子水中,配成1wt%的水溶液,然后在磁力搅拌下,以1ml/min或2ml/min的速度加入乙醇40ml。在加入过程中,溶液变成乳白色,然后在加入就逐渐变清凉,但同时有沉淀出现,请问各位实验过程有啥问题啊?采取哪些措施可以避免出现沉定?另外制备纳米颗粒的过程,对BSA有具体的要求吗
我理解就是一个是包裹,一个是镶嵌
微球说白了就是小颗粒,尺寸是微米级的,再小就是纳米球了,微球可以是纯球,也可以不是规则的球,椭圆的也可以.
聚苯乙烯微球就是利用搅拌乳化,电喷雾,共轴液流(气流),膜乳化等方法将聚苯乙烯制备成微米尺寸的颗粒.
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