请使用支持JavaScript的浏览器! Sh RNA干扰后,mRNA水平干扰不明显,蛋白水平明显,如何解释?相关文献?_蚂蚁淘商城
Sh RNA干扰后,mRNA水平干扰不明显,蛋白水平明显,如何解释?相关文献?
2021-07-20
问题描述:

找公司设计了重组慢病毒载体,共设计3条干扰序列,转染靶细胞测干扰效率。发现mRNA表达水平下降不明显,而蛋白水平明显下降。请问该如何解释,有相关文献吗?可以用抑制了靶蛋白翻译来解释吗?


*请输入10-500个字符
已帮助
0人
mpark
  用户
How is the level of Cbl-b mRNA determined?
已帮助
0人
butterflybow
  用户
实时定量荧光PCR
已帮助
0人
mpark
  用户
ItwouldbebetterthatasingleWesternblotbeusedandshowboth115kDatargetproteinAND37kDaloADIngcontrolinordertounderstandtheshRNAexperimentresultstruly.TheshRNAlentivirusinfectionmaybetoxictoproteinsynthesisininfectedcells,specificallybyshRNA2and3,whiletargetmRNAknockdownbyshRNA1showseffective,butRNAtranscriptionisnotinhibitedaround72hourspostinfectionTheinhibitionofproteinsynthesisbylentiviralvectorinfectioncouldberecoveredinshRNA1andinshRNA2,butnotinshRNA3ninetysixhourspostinfection,atthistimepointitrevealsstrongtargetmRNAknockdownbyshRNA1,moderatedinhibitionbyshRNA2,lesseffectiveinhibitionbyshRNA3.AsuggestionthatacombinedlentivirusinfectionwiththoseproducingshRNA1,andshRNA3andshRNA2bedonetofindbestshRNAcombinationshowingeffectivetargetmRNAknockdownwhileposingnoharmtocellularproteinsynthesis.
相关文章