Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 749/776 |
MW | N/A |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | SuperiorLabelingDyes iFluor™DyesandKits |
Related | Generalproteins LabelingviaAminoGroups |
Spectrum | AdvancedSpectrumViewer |
- Prepareproteinsolution(SolutionA):
Forlabeling50ugprotein(assumingthetargetproteinconcentrationis1mg/mL),mix5μL(10%ofthetotalreactionvolume)ofReactionBuffer(ComponentB)with50uLofthetargetproteinsolution.
Note1:Ifyouhaveadifferenceproteinconcentration,adjusttheproteinvolumeaccordinglytomake~50µgproteinavailableforyourlabelingreaction.
Note2:Forlabeling100ugprotein(assumingthetargetproteinconcentrationis1mg/mL),mix10uL(10%ofthetotalreactionvolume)ofReactionBuffer(ComponentB)with100uLofthetargetproteinsolution.
Note3:Theproteinshouldbedissolvedin1Xphosphatebufferedsaline(PBS),pH7.2-7.4;Iftheproteinisdissolvedinglycinebuffer,itmustbedialyzedagainst1XPBS,pH7.2-7.4,oruseAmiconUltra-0.5,Ultracel-10Membrane,10kDa(cat#UFC501008fromMillipore)toremovefreeaminesorammoniumsalts(suchasammoniumsulfateandammoniumacetate)thatarewidelyusedforproteinprecipitation.
Note4:ImpureantibodiesorantibodiesstABIlizedwithbovineserumalbumin(BSA)orgelatinwillnotbelabeledwell.
Note5:Theconjugationefficiencyissignificantlyreducediftheproteinconcentrationislessthan1mg/mL.Foroptimallabelingefficiencythefinalproteinconcentrationrangeof1-2mg/mLisrecommended. - Runconjugationreaction::
- Addtheproteinsolution(SolutionA)toONEvialoflabelingdye(ComponentA),andmixthemwellbyrepeatedlypipettingforafewtimesorvortexthevialforafewseconds.
Note:Usebothvials(ComponentA)oflabelingdyetolabel100ugproteinbydividingthe100ugproteininto2x50ugproteinandreactingeach50ugproteinwithonevialoflabelingdye.Combinetwovialsforthenextstep. - Keeptheconjugationreactionmixtureatroomtemperaturefor30-60minutes.
Note:Theconjugationreactionmixturecanberotatedorshakenforlongertimeifdesired.
- Addtheproteinsolution(SolutionA)toONEvialoflabelingdye(ComponentA),andmixthemwellbyrepeatedlypipettingforafewtimesorvortexthevialforafewseconds.
- StopConjugationreaction:
- Add5uL(for50ugprotein)or10uL(for100ugprotein)whichis10%ofthetotalreactionvolumeofTQ-DyedQuenchBuffer(ComponentC)intotheconjugationreactionmixture(fromstep2.2),mixthemwell.
- Incubateatroomtemperaturefor10minutes.
- Thelabeledprotein(antibody)isnowreadytouse.
References&Citations | CitationExplorer |
Cube-shapedtheranosticpaclitaxelprodrugnanocrystalswithsurfacefunctionalizationofSPCandMPEG-DSPEforimagingandchemotherapy
Authors:FuqiangGuo,JiajiaShang,HaiZhao,KangrongLai,YangLi,ZhongxiongFan,ZhenqingHou,GuanghaoSu
Journal:ColloidsandSurfacesB:Biointerfaces(2017)
Light/magnetichyperthermiatriggereddrugreleasedfrommulti-functionalThermo-sensitivemagnetoliposomesforprecisecancersynergetictheranostics
Authors:YuxinGuo,YangZhang,JinyuanMa,QiLi,YangLi,XinyiZhou,DanZhao,HuaSong,QingChen,XuanZhu
Journal:JournalofControlledRelease(2017)
Thermo-sensitivehydrogelPLGA-PEG-PLGAasavaccinedeliverysystemforintramuscularimmunization
Authors:XiaoyanWang,YuZhang,WeiXue,HongWang,XiaozhongQiu,ZonghuaLiu
Journal:JournalofBiomaterialsApplications(2017):923--932
Affinity-ControlledProteinEncapsulationintoSub-30nmTelodendrimerNanocarriersbyMultivalentandSynergisticInteractions
Authors:XuWang,ChangyingShi,LiZhang,AlexaBodman,DandanGuo,LiliWang,WalterAHall,StephanWilkens,JuntaoLuo
Journal:Biomaterials(2016)
CarboxymethylDextran-StabilizedPolyethylenimine-Poly(epsilon-caprolactone)Nanoparticles-MediatedModulationofMicroRNA-34aExpressionviaSmall-MoleculeModulatorforHepatocellularCarcinomaTherapy
Authors:XiongweiDeng,ZhaoxiaYin,ZhixiangZhou,YihuiWang,FangZhang,QinHu,YishuYang,JianqingLu,YanWu,WangSheng
Journal:ACSappliedmaterials&interfaces(2016):17068--17079
Click-electronmicroscopyforimagingmetabolicallytaggednonproteinbiomolecules
Authors:JohnTNgo,StephenRAdams,ThomasJDeerinck,DanielaBoassa,FrancesRodriguez-Rivera,SakinaFPalida,CarolynRBertozzi,MarkHEllisman,RogerYTsien
Journal:NatChemBiol(2016):459--465
Design,synthesisandevaluationofVEGF-siRNA/CRSasanovelvectorforgenedelivery
Authors:WenZhao,YifanZhang,XueyunJiang,ChunyingCui
Journal:DrugDesign,DevelopmentandTherapy(2016):3851
MolecularBasisandConsequencesoftheCytochromec-tRNAInteraction
Authors:CuipingLiu,AaronJStonestrom,ThomasChristian,JeongsikYong,RyuichiTakase,Ya-MingHou,XiaoluYang
Journal:JournalofBIOLOGicalChemistry(2016):10426--10436
Determinationoftheactivetransportoffucoidanderivedfromokinawamozukuacrossthehumanintestinalcaco-2cellsasassessedbysize-exclusionchromatography
Authors:TakeakiNagamine,KouHayakawa,KyoumiNakazato,MasahikoIha
Journal:JournalofChromatographyB(2015):187--193
Multiplexedsingle-cellinsituRNAanalysisbyreiterativehybridization
Authors:LuXiao,JiaGuo
Journal:AnalyticalMethods(2015):7290--7295
AAT Bioquest AAT Bioquest是一家位于美国的生物公司,前身为ABD Bioquest,总部位于加利福尼亚州。专门从事光学检测技术十多年,一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术,综合了化学、生物学和信息学等各个领域的研究,引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络,为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。
美国AATBioquestInc.(前身是ABDBioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色),荧光和发光技术。AATBioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。AATBioquest会不断介绍新产品,快速的丰富各个领域的产品。
1)我们提供反应荧光探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物;2)我们研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞。3)我们不断的推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类;4)我们致力于开发用于信号转导研究的试剂;5)我们提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
作为AATBioquestInc.的中国区域代理,艾美捷科技为中国客户提供光谱学检测领域,包括吸收(颜色),荧光和发光技术等全系列解决方案。我们也将一如既往更加努力为国内用户提供快捷、方便的高质量产品,同时更为您售前售后全面技术支持。
AATBioquest,Inc.(formerlyABDBioquest,Inc.)develops,manufacturesandmarketsbioanalyticalresearchreagentsandkitstoscientistsengagedinlifesciencesresearch,diagnosticR&Danddrugdiscovery.Wespecializeintheareaofphotometricdetectionsincludingabsorption(color),fluorescenceandluminescencetechnologies.TheCompany"sproductsenablescientistsandbiomedicalresearcherstobetterunderstandbiochemistry,immunology,cellBIOLOGyandmolecularbiology.AATBioquestconstantlyintroducesnewproducts,andoffersarapidlyexpandinglistofproductsthataregroupedintoseveralproductlines.
1)Ourreactivefluorescentandluminescentprobes,biotinsandtagenzymesareusedforlabelingsmalldrugmoleculesandbiopolymers,e.g,proteins,nucleicacidsandcarbohydrates;2)Wedevelopfluorescentandluminescentprobesfordetectingproteins,nucleicacidsandlivecells;3)Weconstantlyintroducenovelfluorescentandluminescentprobesfordetectingvariousenzymes,inparticular,hydrolyticandredoxenzymes;4)Wefocusondevelopingreagentsforsignaltransductionresearch;and5)Wealsoofferphysiologicalandneurologicalprobes,e.g.,calciumindicatorsandmembranepotentialprobes.
Besidesthestandardcatalogproductswealsooffercustomservicetomeetyourspecialresearchneeds.Ourcurrentservicesincludecustomsynthesisofcolorimetric,fluorescentandluminescentprobes,customdevelopmentofbiochemical,cell-basedanddiagnosticassaysandcustomscreeningofyourcompoundlibrariesagainstyourdefinedtargetsusingourvalidatedHTSassays.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
在弱碱性(pH 8~9)、暗处、室温或40℃条件下,氨基酸的α-氨基很容易与2,4-二硝基氟苯(缩写为FDNB或DNFB)反应,生成黄色的2,4-二硝基苯氨基酸(dinitrophenyl amino acid,简称DNP-氨基酸)。多肽或蛋白质的N-末端氨基酸的α-氨基也能与FDNB反应,生成一种二硝基苯肽(DNP-肽)。由于硝基苯与氨基结合牢固,不易被水解,因此当DNP-多肽被酸水解时,所有肽键均被水解,只有N-末端氨基酸仍连在DNP上,所以产物为黄色的DNP-氨基酸和其它氨基酸的混合液。混合液中只有DNP-氨基酸溶于乙酸乙酯,所以可以用乙酸乙酯抽提并将抽提液进行色谱分析,再以标准的DNP-氨基酸作为对照鉴定出此氨基酸的种类。因此2,4-二硝基氟苯法可用于鉴定多肽或蛋白质的N-末端氨基酸。
荧光标记物常用的有几十种,比如FITC, PE等等,各个生产厂家还有自己的专利产品
不知道发在这里合适不,实在是求助无门啊!版主手下留情。
想请问下那个公司有专门的蛋白质荧光标记试剂盒出售。最好的是CY5的,我准备做三标。性价比越高越好
那些做过的前辈们指导一下。
那为什么SFDA不批准CA199CEAAFP等检测试剂盒作为癌症检测的手段呢?
暂无品牌问答