Product Overview
Background
Urokinase-type Plasminogen Actvator (uPA) is a glycosylated serine protease that is synthesized in endothelial and kidney epithelial cells. There are two forms of uPA, the low molecular weight (LMW ~31 kDa) and high molecular weight (HMW ~55 kDa). The HMW form undergoes autoproteolysis resulting in the LMW plus and 18.5 kDa amino terminal fragment (ATF). It is this ATF that has been shown to inhibit proliferation and invasion of cancer cells by binding to uPA receptors (1).
Assay Principle
This is an ELISA for the quantitative analysis of active uPA levels in biological fluid. This test kit operates on the basis of sandwich ELISA where free active uPA enzyme complexes with PAI-1 and is quantitated with the use of an HRP labeled secondary antibody.Provided is biotinylated PAI-1 bound to avidin coated wells. This allows active tPA present in the standard or unknown, to complex with PAI-1. Inactive or complexed uPA is removed in a subsequent wash step. A primary antibody specific for uPA is then added to each well followed by the HRP conjugated secondary antibody. The bound conjugated secondary antibody is detected by the addition of substrate, which generates an optimal color after 10 minutes. Quantitative test results may be obtained by the measure and comparison of the sample and standard absorbance readings when read with a microplate reader at 450 nm.
Product Documents
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目前选择这个都是北京博凌科为生物科技有限公司的
血液保存液属于医疗器械的几类?
生产单位 天津百利鑫生物科技有限公司
地址
邮编
产品名称 细胞保存液
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从以上药监局已注册的产品信息看,属于第1类
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选,但由于实验室距离医院有3个小时的车程,请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性?谢谢
我搜索了一下网络上的,只找到说要保存在酸性下,但是没有具体的配方。
暂无品牌问答