ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein
- Product No.KSA092Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.62-1000pg/mL
- Applicable Sensitivity5.5pg/mL.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM96T*596T*1096T*2096T*5096T*100
- FOBUS$ 842 For more details, please contact local distributors!US$ 1404 For more details, please contact local distributors!US$ 2527 For more details, please contact local distributors!US$ 4914 For more details, please contact local distributors!US$ 8424 For more details, please contact local distributors!
SPECIFITY of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
The Abs in the kit have high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a).No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA092Hu01 | Recombinant Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA092Hu01 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
APA092Hu61 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
EPA092Hu61 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA092Hu51 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA092Hu07 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
PAA092Hu01 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
PAA092Hu06 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
MAA092Hu22 | Monoclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
SEA092Hu | ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA092Hu | CLIA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Chemiluminescent immunoassay for Antigen Detection. |
KSA092Hu01 | ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Main materials for "Do It (ELISA Kit) Yourself". |
PGA092Hu01 | Primer Pair for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | PCR |
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调节至最适合温度(一般是37度),合适的酸碱度时,酶的活性最高
ELISA实验,操作的时候,加入底物,溶液变成蓝色,孵育一段时间后,加入终止液,变成黄色,测吸光值。然后,当天忘记扔板子了,第二天想到去扔的时候,所有孔都变成无色透明的了!!!为什么会褪色???是试剂有问题?还是因为室温太高环境影响?如果是试剂问题那我之前做的结果还能信么?
1,底物DNA上没有该限制酶的识别、切断位点。特别是一些经过重组等处理的DNA,碱基易发生缺失、变化等。
2,限制酶识别位点上的A或C被甲基化。部分限制酶对识别位点中的碱基是否被甲基化比较敏感,从而无法切断该位点。
3,底物不纯。如果底物DNA中有限制酶阻害物质,回影响限制酶的酶切作用。在此种情况下,底物DNA须重新进行精制。
4,限制酶的识别、切断位点在底物DNA的高级构造中所处的位置,对酶切反应也有一定的影响,例如,限制酶NaeI在切断pBR322DNA时,就有着非常难以切断的部位。
5,限制性内切酶本身无活性或低活性
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