pH:Varies
Conductance:Varies
Appearance:ClearSolutions
Features
- 384Crystallizationconditions,optimized throughhighthroughputvalidation
- Derivedfromtrialsofmorethan40,000diverseproteinsat theMidwestCenterforStructuralGenomics
- Broadcoverageofcrystallizationspace
- Derivedfromcrystallizationoffrombothprokaryoticandeukaryoticorganisms
TheMCSGSuitehasbeendevelopedattheMidwestCenterforStructuralGenomics.AspartoftheProteinStructureInitiative,MCSGhasbeeninstrumentalinthedevelopmentofrapid,integratedandcost-effectivemethodsforproteinstructuredeterminationviaX-raycrystallography.
Throughextensivecrystallizationscreeningofmorethan40,000diverseproteinsfrombothprokaryoticandeukaryoticorganisms,researchersattheMCSGhavederivedthemostup-to-datesetofcrystallizationconditionsforinitialscreening.Thesenon-redundantconditionsconstitutethebroadestcoverageofcrystallizationspaceofanycommerciallyavailablescreenandaresupportedbyadecadeofexperimentalanalysisandvalidation.
ThecompleteMCSGsuite(384conditions)comprisesof4screensof96uniqueconditionsthatareavailableeitherasdeep-wellblocks(1.7mLperwell)ortubes(10ml). Conditions1-288areorganizedinorderofdecreasingcrystalproductivityinscreensMCSG-1,MCSG-2andMCSG-3. ThusMCSG-1:A1conditionhasyieldedthegreatestnumberofuniquecrystalsforalltargetsscreenedwiththesuitepredecessors. TheconditionsthatcompriseMCSG-4havebeenrationallyselectedtosystematicallysampleregionsofcrystallizationspaceforproteinsrequiringadditionalscreening.
Theendresultisthebest,first-passscreeningsuitepossIBLe,with:
- Data-drivenselectionindividualconditionsensuringconditionswithhighproductivitypotentialwerenotaccidentallyexcluded
- Superiororganization
- ThebroadestcoverageofcrystallizationspacewithinareasonablenumberofstartingconditionsformaximalefficiencywhilemaintainingapplicABIlityformoreuniqueanddifficulttargets.
美国Anatrace成立于1984年.全球公认的膜蛋白研究用产品供应商,为膜蛋白提取与纯化提供高纯度生化产品,包括了各种表面活性剂、试剂盒及各种研究用工具书。提供膜蛋白提取和纯化试剂,高纯的生化试剂和清洁剂,去垢剂等
ThermoFisherScientificprovidesinnovativeAffymetrixproducts,tools,andresourcesthathelpadvancetheworkofresearchersviamicroarrayanalysis.Applicationareasthatbenefitfromsuchanapproachincludeplantandanimalgenomicsandtranscriptomics,includingbasicresearchandindustrialapplicationoftechnologiesforbreeding,populationdiversityandconservation,traitanalysis,andmore.Uniquesolutionsarealsoavailableforcancerresearchfromdiscoverytoclinicalresearchandvalidation,suchascytogeneticsresearchofconstitutionalandcancerdisorders.Thegeneticsofhumancomplextraits,Mendeliandisorders,andpopulationsarealsoapplicationarraysthatcanbeadvancedwithAffymetrixmicroarrayanalysis,suchasthroughpopulation-optimizedandapplication-optimizedgenotypingtoenablehumangeneticresearchworkflows. 利用Affymetrix微阵列分析产品推进您的研究 品牌 货号 名称 规格 Anatrace C321 C321-cymal-1 1gm5gm25gm Anatrace C322 C322-CYMAL-2,Anagrade 1gm5gm25gm Anatrace C323 C323-CYMAL-3,Anagrade 1gm5gm25gm Anatrace C323G C323G-CYGLU-3,Anagrade 1gm5gm25gm Anatrace C324 C324-cymal-4,Anagrez 1gm5gm25gm Anatrace C324G C324G-CYGLU-4,Anagrad 1gm5gm25gm Anatrace C325 C325-CYMAL-5,Anagrade 1gm5gm25gm Anatrace C325S C325S-CYMAL-5,Sol-Grade 1gm5gm25gm Anatrace C326 C326-CYMAL-6,Anagrade 1gm5gm25gm Anatrace C326LA C326LA-CYMAL-6,Anagrade 1gm5gm25gm Anatrace C326S C326S-CYMAL-6,Sol-Grade 1gm5gm25gm Anatrace C327 C327-CYMAL-7,Anagrade 1gm5gm25gm Anatrace C327S C327S-CYMAL-7,Sol-Grade 1gm5gm25gmAdvanceyourresearchwithAffymetrixmicroarrayanalysisproducts
ThermoFisherScientific提供创新的Affymetrix产品、工具和资源,帮助通过微阵列分析推进研究人员的工作。受益于这种方法的应用领域包括动植物基因组学和转录组学,包括育种、种群多样性和保护、性状分析等技术的基础研究和工业应用。独特的解决方案也可用于癌症研究,从发现到临床研究和验证,如体质和癌症疾病的细胞遗传学研究。人类复杂性状、孟德尔病和群体的遗传学也是可以通过Affymetrix微阵列分析进行改进的应用阵列,例如通过群体优化和应用优化基因分型来实现人类遗传研究工作流程。
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我用Promega公司的双荧光素酶检测试剂盒(E2920)检测到的firefly萤光素酶活性很低,只有4*10的3次方;海肾萤光素酶活性有10的6次方。
我用Ad293细胞做了转染,fireflyluciferase质粒:RanillaLuciferase质粒=0.1ug:0.025ug/一个孔(96孔板),共转染了二天,再进行双萤光素酶检测。
我想了解fireflyluciferase活性用promega的这个E2920-双萤光素酶试剂盒检测得到10的3次方,这种数值正常吗?
我做了3次重复实验,每次firefly萤光素酶活性很低,只有4*10的3次方;而海肾萤光素酶活性有10的6次方。
求指教?fireflyluciferase活性低?会是什么原因呢?
暂无品牌问答