Description:
TECO®αGSTisanELISAforthequantitativedeterminationofalpha GlutathioneS-Transferase(αGST)inhumanserum,plasmaor urine.
αGST isametabolicenzymeinvolvedwith cellulardetoxificationreactions.Itis approximately51kDaanditcomprises ~5%ofsolublecytosolicproteininhepatocytes.Uponcellulardamage,αGSTisrapidlyreleasedfromcells.Anincreasedconcentrationinbloodisasensitiveindicatorofacutehepaticinjury.StudieshaveshownthatincreasedserumorplasmaαGSTcan beindicativeofhepatotoxicity,allograftrejection,orviralhepatitis.
Inthekidney, αGSTcomprises~2%ofthecytosolicsolubleproteincontent intheepithelialcellsoftheproximaltubule.Likewithhepatocytes,itisrapidlyreleasedupondamagetothe cells.Increasedurineconcentrationsareindicativeofacutekidneyinjury.
Advantages:
- UnlikewithothertoxicitybioMarkers,αGSThasnotbeenshowntoincreasewithmuscledamage,hemolysisorgeneralinflammation(i.e.Rheumatoidarthritis).
- αGSTisrapidlyreleaseduponcellulardamageandthususefulasanearlyindicatorofacuteliver(orkidney)damageinresearchsettings.
- SuitabletousewiththeM30Apoptosense®ELISA(apoptosisdetection),M65®ELISA(necrosisdetection),andTECO®HyaluronicAcidELISA(chronic,fibrosisdetection)asapanelofresearchassaysusefulforassessingliverdamage.
- Applicationsforcellcultureandculturespheroids.
- SandwichELISAwith a96-wellplateinaconvenient,ready-to-useformat.
- Easytoperformassaywithminimalpipettingsteps.
KitComposition:
- AntibodyCoatedMicroassayPlate: 96well(12×8break-apartwellstripscoatedwithIgGdirectedagainstαGST)
- Calibrator,0.2mL(2mg/L): PurifiedαGSTinstABIlizingdiluentcontainingProClin950andBronidoxLaspreservatives.25xConcentrate.
- SampleDiluent,30mL: ProteincontainingsolutionwithaddedstabilizersandProClin950andBronidoxLaspreservatives.
- WashBuffer,45mL: Tris-bufferedsaline/Tween-20(TBST)containingProClin950aspreservative.25xConcentrate.
- PositiveControl,1.5mL: PurifiedαGSTinstabilizingdiluentcontainingProClin950andBronidoxLaspreservatives.
- EnzymeConjugate,11mL: Antibodysolutioncontaininganti-αGSTIgGlabelledwithhorserADIshperoxidaseandProClin950andBronidoxLaspreservatives.
- Substrate,11mL: StabilizedliquidTMBsolution.
- StopSolution,11mL: 0.5MSulphuricAcid
- UrineStabilisingBuffer(USB),10mL: ProteincontainingsolutionwithaddedstabilizersandProClin950andBronidoxLaspreservatives.
AssayPrinciple:
Theα-GSTassayisastandardsandwich ELISA.The96-wellmicroplateiscoatedwithanti-αGSTIgG.Sampleaddedtothewellsforanincubationbindsto the microwell-coatedantibody. Additionofantibody-enzymeconjugatebindsinasandwichfashiontosamplescontainingalpha-GSTandthenTMBsubstrate allowsforcolorimetricdeterminationof αGSTconcentration.Thecolorintensityisdirectlyproprotionaltotheamountof αGSTinasampleandtheassayrangeis 2.5–80μg/L.
Background:
Inliver, αGST islocatedinthehepatocyteswhereaspiGSTisfoundin theintrahepaticbileductcells.ThisheterogeneousGSTsubclassdistributionsuggeststhattheisoenzymeshaveuniqueinvivofunctionsindifferenthepaticregionsandthatthedetectionofGSTsubclasslevelsinBIOLOGicalfluidscouldbeofsignificantuseinmonitoringtheintegrityofspecifichepaticregions.Currently,liverinjuryisstudiedbythemeasurementofliverenzymessuchasalanineaminotransferase(ALT)andaspartateaminotransferase(AST).Adisadvantageofthesemarkersisthattheyarenotdistributeduniformlythroughouttheliverandthattheirperiportalconcentrations are greaterthantheircentrilobularconcentrations.Incontrast,αGSThasbeenfoundtobeequallydistributedinboththecentrilobularandperiportalregionsoftheliver.SincethecentrilobularhepatocytesareverysusceptIBLetodamageinavarietyofconditionsincludingallograftrejection,viralhepatitis,andhepatotoxicity,αGSTisamoresensitiveindicatorofhepaticinjury.The AlphaGSTEIAisaspecific,preciseimmunoassay and,beingaquantitativetest, itisnotaffected bymodulatorsofenzymeactivitysuchas bilesaltsandbilirubin.
Inkidney,αGSTisfoundintheproximaltubuleregion. Inhealthy individuals,lowlevelsofαGSTarereleasedintotheurineasconfirmedbyimmunoassayandWesternblotanalyses.Eventsthatleadto proximaltubulardamageoften causeincreasedreleaseofαGSTintourine.Elevated urinaryαGSTlevelshavebeenshowntoindicate proximaltubuledamagedueto nephrotoxicity,environmentaltoxicity,surgery,acuterenalfailureandtransplantation.
αGSTisoftendescribedasa“leakage”biomarkersinceitisrapidlyreleasedfromdamagedcells.ThoughintendedforresearchuseonlyintheUSandCanada,theAlphaGSTEIAcanbeausefulindicatorofacuteliver(orkidney)damageandisthusausefulresearchassayforassessingtoxicity.TheTECOAlphaGSTEIAcanalsobeusedwithhumanlivercellculturesamples,providinganapplicationfor invitrodrugscreening.
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通过解答,教会我,谢谢
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
刚入这行,谢谢大家
我想检测血管组织中的钙离子浓度,不知道哪个公司有试剂盒
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
暂无品牌问答