Description:
Technozym®tPA-PAI-1ComplexELISAisaresearchassay kitfordeterminationoft-PA-PAI-1complexesinplasma.Thet-PA-PAI-1Complexkitisbasedonamonoclonalantibodydirectedagainstt-PAasacatchingantibodyfort-PA-PAI-1complexesandaperoxidase-labeledmonoclonalanti-PAI-1antibodyusedforthedetectionofboundt-PA-PAI-1complexes. TheTechnozym®t-PA-PAI-1testisasolidphaseenzymeimmunoassay.
KitComposition:
- PLATE+PLATECOVER:12×8wellmicrotitrestripsprecoatedwithamonoclonalantit-PAcoatingantibodyinbicarbonatebufferandblockedwith1%bovineserumalbumin(BSA)(TC-CodeGN).
- STANDARD:1xlyophilizedt-PA-PAI-1complexes(TC-CodeBT)
- POX-ANTIBODY:1xconjugatedmonoclonalanti-PAI-1antibody(concentrated)(TC-CodeKL)
- DILUTIONBUFFER: (whitecap)1x20ml2.5xconcentrated(PBS,1%BSA,5mMEDTA(TC-CodeAD).
- POXDILUTIONBUFFER: (whitecap)1x12mlPBS,1%BSA(TC-CodeDD).
- SUBSTRATE: (greencap)1x12mLTMB(Tetramethylbenzidine)insubstratebuffercontainingH2O2.Readytouse.(TC-CodeKN)
- STOPSOLUTION:1x15mlsulfuricacid0,45mol/l(TC-CodeKK)
- WASHBUFFER: (bluecap)1x20ml12×5xconcentrated(PBS0.5%,Tween20)(TC-CodeBE)
Background:
PlasminogenActivatorInhibitorTypeI(PAI-1)isthemajorinhibitoroftissuetypeplasminogenactivator(t-PA).Intherestingstate,thebody’splasmaconcentrationofPAI-1greatlyexceedsthet-PAplasmalevelandevenfollowingphysicalexertionorvenousocclusion,conditionswhichleadtoincreasedt-PAlevels,PAI-1isusuallyavailableinadequateamountstocompensateforincreasedactivatorlevels.Thus,increasesint-PA-PAI-1complexesaslongasactive,non-complexedPAI-1isavailable.Certainpathologicalconditionsareassociatedwithincreasedt-PAvalues.Theliveristhemajorsiteoft-PAdegradationandhepaticdisordersareoftenaccompaniedbyincreasedt-PAlevelsand,thus,increasedt-PA-PAI-1complexvalues.
Inhealthypersons,thet-PAlevelincreaseswithageandcomplexlevelsriseaccordingly.Thequantitationoft-PA-PAI-1complexesaidsintheinterpretationofalterationsinthelevelsoft-PAand/orPAI-1.Sincetheliver,inadditiontobeingasiteoft-PAdegradation,isanorganofPAI-1synthesisthequantitationofactivator-inhibitorcomplexesmayserveasandindicatoroftheextenttowhichactivePAI-1isavailabletocompensateforareductioninthehepaticdegradationoft-PA,i.e.elevationoft-PAplasmavalues.
Thedetectionofelevatedt-PA-PAI-1complexvaluesmaybeusefulfortheresearch ofliverdysfunctionswhichmayresultinclottingdisorders.
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
暂无品牌问答