Description:
REAADSAnti-ProthrombinIgM isan anti-ProthrombinantibodyELISAtestkitforthe semi-quantitative,indirectdetectionofantibodiesagainsthumanprothrombin.AsinglepointcalibratorisusedtodetermineIgManti-prothrombinantibodyconcentrationsinhumanserumofcitratedplasma.
Features:
- ConvenientELISAprocedure
- ForthespecificdeterminationofIgMisotype
- Objective,accurateandreproducIBLe
- Reagentcompletekits
- Totalincubationtime:40minutesatroomtemperature
PrincipleandProcedure:
Principle
TheREAADSIgManti-Prothrombinantibodytestkitsaresemiquantitative,indirectELISAsforthedetectionofantibodiesagainsthumanprothrombin.AsinglepointcalibratorisusedtodetermineIgManti-prothrombinantibodyconcentrationsinhumanserumofcitratedplasma.
Procedure
Dilutedpatientserumorplasmaisincubatedinmicrowellscoatedwithhumanprothrombin.Anti-Prothrombin(aPT)antibodiesinpatientsampleswillbindtotheimmobilizedantigeninthewells.Afterwashingtoremoveunboundserumorplasmaproteins,antibodiesspecificforhumanIgM,labeledwithhorserADIshperoxidase(HRP),areboundantibodies.Thewellsarewashed,andachromogenicsubstrateisadded,resultinginacolorchangethatisproportionaltotheamountofantibodypresent.Theassayiscompletedbytheadditionofastoppingsolution.ResultsareobtainedbyreadingtheO.D.(opticaldensityorabsorbance)at450nmofeachwellinaspectrophotometer.TheO.D.valuesofcontrolandpatientsamplesaremultipliedbythecalibratorconversionfactortoobtainIgMaPTantibodyconcentrationstimeis40minutesatroomtemperature.
ClinicalPerformance*:
ClinicalSpecificity:serumsamplesfrom100healthyblooddonorsweretestedforaPTantibodies.Againstanestablishedcutoffvalueof20Gand20Munits,thespecificityoftheIgMassaywas97%.Aspresentedinthetablebelow,similarresultswereseenwhenplasmasamplesfrom100healthyblooddonorsweretested.Assayspecificitywith42infectiousdisease(syphilis)patientsand42RheumatoidArthritis(RA)patientsisalsopresentedinthetable.
ClinicalSensitivity:serumsamplesfrom41unselectedSLEpatientsweretestedforIgMaPTantibodies.14.6%ofthesampleswerepositiveforIgMaPTantibodies(meanvaluesof11.8Munits).Serumsamplesfrom11selectedfemalepatientswithprimaryanti-phospholipidsyndrome(APS)werealsoevaluatedforantibodiestoprothrombin.27.3%werepositiveforIgMantibodies(meanvaluesof15.7Munits).
IgMaPT (%positive) | |
Healthyserum (n=100) | 3% |
Healthyplasma (n=100) | 3% |
Syphilis (n=42) | 11.9% |
RA (n=42) | 4.8% |
SLE (n=41) | 12.2% |
APS (n=11) | 27.3% |
*Dataavailableuponrequest.
Background:
Severalplasmaproteinsassociatedwiththecoagulationsystemandwithstrongphospholipidbindingpropertieshavebeenidentifiedasantiphospholipidcofactors.Antibodiestoprothrombin,oneoftheseplasmaproteins,havebeenreportedinpatientswithantiphospholipidsyndrome.TestingfortheseantibodiesisproposedtobeincludedintheSEROlogicevaluationofantiphospholipidantibodies.Clinically,elevatedserumlevelsofanti-prothrombin(aPT)antibodiesareassociatedwithanincreasedriskforAPS,characterizedbyrecurrentarterialorvenousthrombosis,thrombocytopeniaand/orfetalloss.HighserumorplasmalevelsofaPTantibodiesmayaddvaluableinformationinthelaboratoryassessmentofantiphospholipidantibodies.
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
暂无品牌问答