Description:
REAADSAnti-Cardiolipin(aCL)IgG/IgM is an ELISAtestkitforthedeterminationof IgG/IgManti-cardiolipin antibodiesinhumanserumorplasma inindividualswithsystemiclupus erythematosus(SLE)andlupus-likedisorders(anti-phospholipidsyndrome).
KitComposition:
Reagents,StorageandStABIlity
- Stabilizedbeefheartcardiolipin(diphosphatidylglycerol)coatedmicrowells:96(12stripsof8breakawaywells),withframe.
- SampleDiluent:1bottle(60ml)(greensolution),containsbovinecalfserum. Containssodiumazide.
- aCLIgGCalibratorSera(1-high,2-moderate,3-low)(human): 3vials(0.250ml)seeviallabelforantibodyconcentrationinGPLunits.Calibrator2shouldbeusedwhenperformingsinglepointcalibration. Containssodiumazide.
- aCLIgMCalibratorSera(1-high,2-moderate,3-low)(human): 3vials(0.250ml)seeviallabelforantibodyconcentrationinMPLunits.Calibrator2shouldbeusedwhenperformingsinglepointcalibration. Containssodiumazide.
- aCLIgGPositiveControlSerum(human): 1vial(0.250ml)seeviallabelforexpectedGPLrange. Containssodiumazide.
- aCLIgMPositiveControlSerum(human): 1vial(0.250ml)seeviallabelforexpectedMPLrange. Containssodiumazide.
- aCLNormalControlSerum(human): 1vial(0.250ml)seeviallabelforexpectedGPLandMPLranges. Containssodiumazide.
- anti-humanIgG(goat)HRP-ConjugatedAntibodySolution:1bottle(15ml)(bluesolution).
- anti-humanIgM(goat)HRP-ConjugatedAntibodySolution:1bottle(15ml)(redsolution).
- OneComponentSubstrate(TMBandH2O2): 1bottle(15ml)readytouse.
- StoppingSolution: 1bottle(15ml)(0.36Nsulfuricacid).
- WashConcentrate:2bottles(30ml) (33XPBS).
Storeat2–8°C.DoNotFreeze.
MeasurementPrinciple:
ThetestisperformedasanindirectELISA.Dilutedserumsamples,calibratorsera,andcontrolsareincubatedincardiolipincoatedmicrowells,allowingaCLantibodiespresentinthesamplestoreactwiththeimmobilizedantigen.Aftertheremovalofunboundserumproteinsbywashing,antibodiesspecificforhumanIgGorIgMlabeledwithhorserADIshperoxidase(HRP)areaddedformingcomplexeswiththecardiolipinboundantibodies.Twoenzymeconjugatedantibodysolutionsareprovided,onespecificforhumanIgGantibodiesandonespecificforhumanIgMantibodies.TheconcentrationofIgGaCLantibodiesandIgMaCLantibodiesmustbedeterminedseparately.Followinganotherwashingstep,theboundenzyme-antibodyconjugateisassayedbytheadditionoftetramethylbenzidine(TMB)andhydrogenperoxide(H2O2)asthechromogenicsubstrate.ColordevelopsinthewellsatanintensityproportionaltotheserumconcentrationofaCLantibodies.ResultsareobtainedbyreadingtheO.D.(opticaldensityorabsorbance)ofeachwellwithaspectrophotometer.Calibratorseraareprovided,withtheIgGandIgMaCLconcentrationsexpressedinGPLorMPLunits,respectively.TheseunitsaretraceabletotheinternationallyrecognizedpreparationsofthePhospholipidStandardizationLaboratory,UniversityofLouisville.Theuserhastheoptionofrunningeitherasinglepointcalibratororafour-pointcalibrationcurve.Forsinglepointcalibration,dividingtheconcentrationvalueofthecalibratorserabytheO.D.valueofthecalibratorprovidesaconversionfactor(oneforIgGandoneforIgMaCL).TheO.D.valuesofalltheothersamplesaremultipliedbytheconversionfactorstoobtainIgGandIgMaCLantibodyconcentrationsinstandardunits.OneGPLunitisequivalentto1µg/mLofanaffinitypurifiedstandardIgGsample,andoneMPLunitisequivalentto1µg/mLofanaffinitypurifiedstandardIgMsample.Formultipointcalibration,performalinearregressionanalysiswithcalibratorvaluesagainstcalibratorO.D.s.Controlandpatientresultsaredeterminedfromthecalibrationcurve.
KitSizes:
CATALOG# | TYPE | WELLS | FORMAT |
K023-001 | IgG/IgM | 96 | rapidformat(15-15-10) |
K023-002* | IgG/IgM | 288 | rapidformat(15-15-10) |
K026-001 | IgA | 96 | rapidformat(15-15-10) |
K026-006* | IgA | 288 | rapidformat(15-15-10) |
K11139* | IgG | 96 | extendedincubations(30-30-30) |
K12747* | IgG | 288 | extendedincubations(30-30-30) |
K11140* | IgM | 96 | extendedincubations(30-30-30) |
K12748* | IgM | 288 | extendedincubations(30-30-30) |
K11141* | IgA | 96 | extendedincubations(30-30-30) |
K12770* | IgA | 288 | extendedincubations(30-30-30) |
*Thisproductmayrequireaspecialorder.Pleaseinquireatinfo@Diapharma.comor1-800-526-5224.
Background:
Anti-cardiolipin(aCL)antibodiesareassociatedwiththepresenceofbothvenousandarterialthrombosis,thrombocytopenia,andrecurrentfetalloss.Theseautoantibodiesarefrequentlyfoundinpatientswithsystemiclupuserythematosus(SLE)andotherautoimmunediseases,aswellasinsomeindividualswithnoapparentpreviousunderlyingdisease.
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通过解答,教会我,谢谢
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
刚入这行,谢谢大家
我想检测血管组织中的钙离子浓度,不知道哪个公司有试剂盒
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
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