GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
RecombinantHuman Chemokine (C-X-C motif) Ligand 12, Alpha (CXCL12α)StromalCell-Derived Factor 1 (SDF1)SmallInducible Cytokine Subfamily B Member 12 (SCYB12)Pre-B CellGrowth-Stimulating Factor (PBSF)Thymic LymphomaCell-Stimulating Factor (TLSF)
CXCL12 is a CXC chemokine lacking the ELR motif. It was first found in bone marrow stromal cells and is expressed in a wide variety of cells with the exception blood cells. CXCL12 acts as a ligand for CXCR4. It is chemotactic for human hematopoietic progenitor cells expressing CD34, mononuclear cells, and cerebellar granule cells. CXCL12 plays a role in lymphopoiesis, myelopoiesis, vascularization of the gastrointestinal tract, and cerebellar development. CXCL12-alpha is one of six alternatively spliced isoforms of the CXCL12 gene. It is four amino acids shorter at the C-terminus than the canonical sequence, CXCL12-beta.
Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.
Source: Escherichia coli
MW: ~8.0 kDa (68 aminoacids)
Purity: >97% by SDS-PAGEand HPLC analyses
Biological Activity: Fully biologicallyactive when compared to standard. The biological activity determined by achemotaxis bioassay using PHA and rHuIL-2 activated human peripheral bloodT-lymphocytes is in a concentration range of 20-80 ng/ml.
Storage/Handling: Store desiccated at-20°C.
HGNC: 10672
NOT FOR HUMAN USE!
Additional resources for this protein may be found at:OMIM
GoldBio活体成像技术:早在1999年由美国哈佛大学Weissleder博士率先提出了分子影像学(molecularimaging,MI)的概念,即应用影像学的方法对活体状态下的生物过程进行细胞和分子水平的定性和定量研究。活体成像便是基于分子影像学孕育而生的,通过这个成像系统,可以观测活体动物体内肿瘤的生长及转移,感染性疾病的发展进程,特定基因的表达等生物学过程。活体成像技术主要采用生物发光(bioluminescence)与荧光(fluorescence)两种技术。★生物发光是用荧光素酶基因标记细胞或DNA。★荧光技术则采用荧光报告基团(GFP、RFP,Cyt及dyes等)进行标记。★这一技术对肿瘤微小转移灶的检测灵敏度极高,不涉及放射性物质和方法,非常安全。操作极其简单、所得结果直观、灵敏度高。
活体成像两种检测技术介绍活体成像特点优点缺点生物发光检测bioluminescence★荧光素酶(Luciferase)对基因、细胞和活体动物进行标记;★荧光素酶催化底物(例如荧光素钾盐)反应后,会产生化学发光。这种光是由化学反应而来,不需要激发光;★标记方法是通过克隆技术,将荧光素酶的基因插入到预期观察的细胞染色体内,通过对克隆细胞进行筛选,培养出能稳定表达荧光素酶的细胞株。再将细胞株转移至特定的小鼠体内形成模型。★特异性强,无自发荧光;★高灵敏度,在体内可检测到几百个细胞,检测的深度在3-100px;★定量精确 ★信号较弱,检测时间较长;★仪器精密度要求较高;★细胞或基因需要转基因标记;★不可用于人体,不适用于抗体、多肽等标记荧光检测fluorescence★采用荧光报告基因(GFP、RFP等)或荧光染料进行标记;★需要外接激发光源,利用报告基因、荧光蛋白质或染料产生的荧光,就可以形成体内的生物光源。★荧光染料、蛋白标记能力强;★信号强,成像速度快,操作简便,实验成本较低;★未来可用于人;★适用范围广,可以是动物、细胞、微生物,也可以是抗体、药物、纳米材料等。★存在自发荧光,影响灵敏度;★光容易被动物组织吸收;★检测深度受限;★背景光干扰,定量准确度低
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D,K2SO4Na2CO3BaCL2NaNO3
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
通过解答,教会我,谢谢
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