PepMix™Human(MAGEC1)
ProductCode:PM-MAGEC1
Poolof283peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughMelanoma-associatedantigenC1(MAGEC1,Swiss-ProtID:O60732)ofHomosapiensforTcellassays(e.g.ELISPOT).
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Cancer,Melanoma
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
PepMix™Human(MAGEC1)
SelectedReferences
"DysfunctionofPSA-specificCD8+TCellsinProstateCancerPatientsCorrelateswithCD38andTim-3Expression"
Jappetal.,CancerImmunolImmunother.(2015)-PMID:26289091
"AnHPV-E6/E7ImmunotherapyPlusPD-1CheckpointInhibitionResultsinTumorRegressionandReductioninPD-L1Expression"
Riceetal.,CancerGeneTherapy(2015)-PMID:26337747
"Survivin-specificCD4+TCellsareDecreasedinPatientswithSurvivin-positiveMyeloma"
Lockeetal.,JImmunotherCancer(2015)-PMID:25992291
"APhaseITrialCombiningDecitabine/dendriticCellVaccineTargetingMAGE-A1,MAGE-A3andNY-ESO-1forChildrenwithRelapsedorTherapy-RefractoryNeuroblastomaandSarcoma"
Krishnadasetal.,CancerImmunolImmunother.(2015)-PMID:26105625
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"PresenceofCirculatingHer-2-reactiveCD8+T-CellsisAssociatedwithLowerFrequenciesofMyeloid-derivedSuppressorCellsandRegulatoryTCells,andBetterSurvivalinElderlyBreastCancerPatients"
KiniBailuretal.,BreastCancerResearch(2015)-PMID:n.a.
"STAT3SignalingContributestotheHighEffectorActivitiesofInterleukin-15-derivedDendriticCells"
Okadaetal.,ImmunolCellBiol.(2015)-PMID:25582338
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
PepMix™Human(MAGEC1)
TechnicalData:Application | AntigenspecificT-cellstimulation CancerbioMarkerresearch Immunemonitoring T-cellassays T-cellexpansion |
ProteinName | Melanoma-associatedantigen(MAGE) |
Organism | Homosapiens(Human) |
Indication/Topic | Cancer Melanoma |
Numberofpeptides | 283 |
FurtherInformation:
Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
Specifications | Peptidescan(15merswith11aaoverlap) |
Documentation | ProtocolandDatasheet |
Swiss-ProtID | O60732 |
Sequence | MGDKDMPTAGMPSLLQSSSESPQSCPEG EDSQSPLQIPQSSPESDDTLYPLQSPQS RSEGEDSSDPLQRPPEGKDSQSPLQIPQ SSPEGDDTQSPLQNSQSSPEGKDSLSPL EISQSPPEGEDVQSPLQNPASSFFSSAL LSIFQSSPESTQSPFEGFPQSVLQIPVS AASSSTLVSIFQSSPESTQSPFEGFPQS PLQIPVSRSFSSTLLSIFQSSPERTQST FEGFAQSPLQIPVSPSSSSTLLSLFQSF SERTQSTFEGFAQSSLQIPVSPSFSSTL VSLFQSSPERTQSTFEGFPQSPLQIPVS SSSSSTLLSLFQSSPERTHSTFEGFPQS LLQIPMTSSFSSTLLSIFQSSPESAQST FEGFPQSPLQIPGSPSFSSTLLSLFQSS PERTHSTFEGFPQSPLQIPMTSSFSSTL LSILQSSPESAQSAFEGFPQSPLQIPVS SSFSYTLLSLFQSSPERTHSTFEGFPQS PLQIPVSSSSSSSTLLSLFQSSPECTQS TFEGFPQSPLQIPQSPPEGENTHSPLQI VPSLPEWEDSLSPHYFPQSPPQGEDSLS PHYFPQSPPQGEDSLSPHYFPQSPQGED SLSPHYFPQSPPQGEDSMSPLYFPQSPL QGEEFQSSLQSPVSICSSSTPSSLPQSF PESSQSPPEGPVQSPLHSPQSPPEGMHS QSPLQSPESAPEGEDSLSPLQIPQSPLE GEDSLSSLHFPQSPPEWEDSLSPLHFPQ FPPQGEDFQSSLQSPVSICSSSTSLSLP QSFPESPQSPPEGPAQSPLQRPVSSFFS YTLASLLQSSHESPQSPPEGPAQSPLQS PVSSFPSSTSSSLSQSSPVSSFPSSTSS SLSKSSPESPLQSPVISFSSSTSLSPFS EESSSPVDEYTSSSDTLLESDSLTDSES LIESEPLFTYTLDEKVDELARFLLLKYQ VKQPITKAEMLTNVISRYTGYFPVIFRK AREFIEILFGISLREVDPDDSYVFVNTL DLTSEGCLSDEQGMSQNRLLILILSIIF IKGTYASEEVIWDVLSGIGVRAGREHFA FGEPRELLTKVWVQEHYLEYREVPNSSP PRYEFLWGPRAHSEVIKRKVVEFLAMLK NTVPITFPSSYKDALKDVEERAQAIIDT TDDSTATESASSSVMSPSFSSE |
ProteinLength(aa) | 1142 |
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通过解答,教会我,谢谢
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
刚入这行,谢谢大家
我想检测血管组织中的钙离子浓度,不知道哪个公司有试剂盒
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
暂无品牌问答