TheDurhampHScreenMD1-101
TheDurhampHScreenisaproteinstABIlityscreendesignedbyEhmkePohl,MortenGrøftehaugeandEmilyCardewatDurhamUniversitytobeusedwithaproteinstabilityassay,suchasthethermalshiftassay(alsoknownasThermoFluor®).ThescreenidentifieshowbufferchemistryandpHaltersthethermaldenaturationandthereforestabilityofaprotein.Inaddition,buffermoleculescanactaslowaffinityligandsandbindtospecificpocketsonaprotein,thiscanbeanundesirableconfoundingfactorinfragment-baseddrugdesignprojects.Therefore,aproteinstabilityscreenfortheoptimumbufferandpHforyoursampleisavitalfirststepinanystructuredeterminationproject.
TheDurhampHScreenincludes28differentbufferscoveringabroadpHrange(4-11),andseparatestheeffectsofbufferchemistryandpH.Werecommendit"susetogetherwiththeDurhamSaltandRUBICsscreenstothoroughlyunderstandtheeffectofproteinenvironmentonstability.
TheDurhamScreensaredesignedtoworkwithNAMI*thefreetodownloadsoftwareforeasyinterpretationofthermalshiftassayresults.
Reference:Grøftehauge,MK,Hajizadeh,NR,Swann,MJandPohl,E.Protein-ligandinteractionsinvestigatedbythermalshiftassays(TSA)anddualpolarizationinterferometry(DPI).ActaCrystD71:36-44(2015).
TheDurhampHScreenispresentedas96x0.5mLconditionsinadeep-wellblock.Theyarepreservativefreeanditisrecommendedthattheyarestoredat4°C,howevertheycanbeusedatroomtemperatureforscreenset-up.
*NAMIcangeneratewaterfallplotsofthermalshiftdataatmultipleconcentrationsorplotsofmeltingtemperatureagainstconcentration.Thesedifferentiateasuddenincreaseinproteinstabilityindicatingaspecificinteractionandsteadyincreasesinstabilityduetothechangingproteinenvironment.Inaddition,itproducesausefulcolourgrADIentplatediagramtomakeidentifyingparticularlystabilisingordestabilisingconditionssimple.
ThermoFluorisaregisteredtrademarkofJohnsonandJohnson.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
暂无品牌问答